{"doi":"10.7717/peerj.2691","title":"Factors influencing infection and transmission of<i>Anopheles gambiae</i>densovirus (AgDNV) in mosquitoes","abstract":"<jats:p><jats:italic>Anopheles gambiae</jats:italic>densovirus (AgDNV) is a potential microbial agent for paratransgenesis and gene transduction in<jats:italic>An. gambiae</jats:italic>, the major vector of human malaria in sub-Saharan Africa. Understanding the interaction between AgDNV and<jats:italic>An. gambiae</jats:italic>is critical for using AgDNV in a basic and applied manner for<jats:italic>Anopheles</jats:italic>gene manipulation. Here, we tested the effects of mosquito age, sex, blood feeding status, and potential for horizontal transmission using an enhanced green fluorescent protein (EGFP) reporter AgDNV system. Neither mosquito age at infection nor feeding regime affected viral titers. Female mosquitoes were more permissive to viral infection than males. Despite low viral titers, infected males were able to venereally transmit virus to females during mating, where the virus was localized with the transferred sperm in the spermathecae. These findings will be useful for designing AgDNV-based strategies to manipulate<jats:italic>Anopheles gambiae</jats:italic>.</jats:p>","journal":"PeerJ","year":2016,"id":14809,"datarank":0.7016859615841454,"base_score":3.1780538303479458,"endowment":3.1780538303479458,"self_citation_contribution":0.47670807455219194,"citation_network_contribution":0.2249778870319535,"self_endowment_contribution":0.47670807455219194,"citer_contribution":0.2249778870319535,"corpus_percentile":null,"corpus_rank":null,"citation_count":23,"citer_count":13,"citers_with_citation_signal":8,"citers_with_endowment":8,"datacite_reuse_total":12,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":115403,"name":"Yasutsugu Suzuki","orcid":"0000-0002-8202-3032","position":1,"is_corresponding":false},{"id":115404,"name":"Jason L. 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Barik","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.1780538303479458,"endowment":3.1780538303479458,"datacite_reuse_total":12,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27867767","pmcid":"PMC5111888","openalex_id":"https://openalex.org/W2550668982","authors":[],"funders":[{"funder_name":"NIH","grant_id":"R21AI088311, R21AI128918, R21AI111175, and R01AI116636","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI116636","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R21 AI088311","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R21 AI111175","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R21 AI128918","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R21AI111175-02","title":"ReMOT Control of mosquito transgenesis"},{"funder_name":"National Institutes of Health","grant_id":"1R21AI088311-01","title":"Efficient site-specific genetic engineering of the Anopheles gambiae genome"},{"funder_name":"National Institutes of Health","grant_id":"1R01AI116636-01A1","title":"Wolbachia-induced enhancement of human arboviral pathogens"},{"funder_name":"National Institutes of Health","grant_id":"5R21AI128918-02","title":"Potential for mosquitoes in the United States to transmit Zika virus"}],"total_grants":9,"fwci":1.5876,"citation_percentile":0.85681259,"influential_citations":0,"citation_trend":[{"year":2017,"count":1},{"year":2018,"count":2},{"year":2019,"count":1},{"year":2020,"count":2},{"year":2022,"count":2},{"year":2023,"count":13},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://peerj.com/articles/2691.pdf","host_type":"journal"},{"url":"https://peerj.com/articles/2691.pdf","host_type":"GOLD"},{"url":"https://peerj.com/articles/2691.pdf","host_type":"publisher"},{"url":"https://peerj.com/articles/2691.xml","host_type":"publisher"},{"url":"https://peerj.com/articles/2691.html","host_type":"publisher"},{"url":"https://doi.org/10.7717/peerj.2691","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27867767","host_type":"repository"},{"url":"https://doaj.org/article/93cc88c46d3d429f94777dfe605b6da3","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5111888","host_type":"repository"},{"url":"http://doi.org/10.7717/peerj.2691","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC5111888","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC5111888?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.7717/peerj.2691","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/27867767/","host_type":""},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC5111888/","host_type":""},{"url":"https://dx.doi.org/10.7717/peerj.2691","host_type":""}],"fields_of_study":["Insect symbiosis and bacterial influences","Mosquito-borne diseases and control","CRISPR and Genetic Engineering","Biology","Medicine","Environmental Science","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Anopheles gambiae","Biology","Anopheles","Virology","Malaria","Vector (molecular biology)","Transmission (telecommunications)","Transduction (biophysics)","Virus","Gene","Genetics","Immunology","Gene transduction","Disease control","Vector-borne Disease","Paratransgenesis","Sex-specific Differences","QH301-705.5","R","Medicine","Biology (General)","Entomology"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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