{"doi":"10.7554/elife.98405","title":"A split-GAL4 driver line resource for Drosophila neuron types","abstract":"Techniques that enable precise manipulations of subsets of neurons in the fly central nervous system (CNS) have greatly facilitated our understanding of the neural basis of behavior. Split-GAL4 driver lines allow specific targeting of cell types in Drosophila melanogaster and other species. We describe here a collection of 3060 lines targeting a range of cell types in the adult Drosophila CNS and 1373 lines characterized in third-instar larvae. These tools enable functional, transcriptomic, and proteomic studies based on precise anatomical targeting. NeuronBridge and other search tools relate light microscopy images of these split-GAL4 lines to connectomes reconstructed from electron microscopy images. The collections are the result of screening over 77,000 split hemidriver combinations. Previously published and new lines are included, all validated for driver expression and curated for optimal cell-type specificity across diverse cell types. In addition to images and fly stocks for these well-characterized lines, we make available 300,000 new 3D images of other split-GAL4 lines.","journal":"eLife","year":2024,"id":484056,"datarank":0.46594100248594855,"base_score":2.995732273553991,"endowment":2.995732273553991,"self_citation_contribution":0.4493598410330987,"citation_network_contribution":0.016581161452849887,"self_endowment_contribution":0.4493598410330987,"citer_contribution":0.016581161452849887,"corpus_percentile":59.2326139088729,"corpus_rank":5271,"citation_count":19,"citer_count":10,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":true,"is_dataset_confidence":0.6454,"is_data_producer":true,"deposit_databanks":{"figshare":["10.25378/janelia.12159378.v2","10.25378/janelia.23726103.v1"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":54.1667,"fair_percentile":68.66401712014674,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1326226,"name":"Allison Vannan","orcid":null,"position":1,"is_corresponding":false},{"id":554933,"name":"Jennifer Jeter","orcid":null,"position":2,"is_corresponding":false},{"id":1326227,"name":"Kari Close","orcid":null,"position":3,"is_corresponding":false},{"id":554930,"name":"Gina M DePasquale","orcid":null,"position":4,"is_corresponding":false},{"id":553788,"name":"Zachary Dorman","orcid":"0000-0001-9933-7217","position":5,"is_corresponding":false},{"id":554931,"name":"Kaitlyn Forster","orcid":null,"position":6,"is_corresponding":false},{"id":1326228,"name":"Jaye Anne Beringer","orcid":null,"position":7,"is_corresponding":false},{"id":553789,"name":"Theresa V Gibney","orcid":"0000-0001-5461-724X","position":8,"is_corresponding":false},{"id":554932,"name":"Joanna H Hausenfluck","orcid":null,"position":9,"is_corresponding":false},{"id":334972,"name":"Yisheng He","orcid":"0000-0002-2217-6758","position":10,"is_corresponding":false},{"id":884889,"name":"Kristin Henderson","orcid":"0000-0002-9265-7709","position":11,"is_corresponding":false},{"id":554934,"name":"Lauren Johnson","orcid":null,"position":12,"is_corresponding":false},{"id":554935,"name":"Rebecca M. Johnston","orcid":null,"position":13,"is_corresponding":false},{"id":1325594,"name":"Gudrun Ihrke","orcid":"0000-0003-4604-735X","position":14,"is_corresponding":false},{"id":231065,"name":"Nirmala Iyer","orcid":"0000-0003-0202-4746","position":15,"is_corresponding":false},{"id":1326229,"name":"Rachel Lazarus","orcid":null,"position":16,"is_corresponding":false},{"id":553791,"name":"Kelley Lee","orcid":"0000-0002-3625-1915","position":17,"is_corresponding":false},{"id":1325595,"name":"Hsing-Hsi Li","orcid":"0009-0001-9883-8201","position":18,"is_corresponding":false},{"id":1325596,"name":"Hua-Peng Liaw","orcid":"0009-0003-4721-3556","position":19,"is_corresponding":false},{"id":554936,"name":"Brian Melton","orcid":null,"position":20,"is_corresponding":false},{"id":1325597,"name":"Scott D. Miller","orcid":"0000-0002-6110-4482","position":21,"is_corresponding":false},{"id":788202,"name":"Reeham Motaher","orcid":"0000-0001-5456-4491","position":22,"is_corresponding":false},{"id":1326230,"name":"Alexandra Novak","orcid":null,"position":23,"is_corresponding":false},{"id":1325598,"name":"Omotara Ogundeyi","orcid":"0000-0003-2770-0026","position":24,"is_corresponding":false},{"id":990716,"name":"Alyson Petruncio","orcid":"0000-0002-1093-6182","position":25,"is_corresponding":false},{"id":1326231,"name":"Jacquelyn Price","orcid":null,"position":26,"is_corresponding":false},{"id":1326232,"name":"Sophia Protopapas","orcid":null,"position":27,"is_corresponding":false},{"id":1326233,"name":"Susana Tae","orcid":null,"position":28,"is_corresponding":false},{"id":313816,"name":"Jennifer Taylor","orcid":"0000-0001-6942-1696","position":29,"is_corresponding":false},{"id":1326234,"name":"Rebecca Vorimo","orcid":null,"position":30,"is_corresponding":false},{"id":554937,"name":"Brianna Yarbrough","orcid":null,"position":31,"is_corresponding":false},{"id":1326235,"name":"Kevin Xiankun Zeng","orcid":null,"position":32,"is_corresponding":false},{"id":553792,"name":"Christopher T Zugates","orcid":"0000-0003-1882-3665","position":33,"is_corresponding":false},{"id":586760,"name":"Heather Dionne","orcid":"0000-0001-7700-8885","position":34,"is_corresponding":false},{"id":1326236,"name":"C. N. Angstadt","orcid":null,"position":35,"is_corresponding":false},{"id":1326237,"name":"Kelly Ashley","orcid":null,"position":36,"is_corresponding":false},{"id":1325599,"name":"Amanda Cavallaro","orcid":"0000-0002-0650-4651","position":37,"is_corresponding":false},{"id":105859,"name":"Tam Dang","orcid":"0000-0002-3602-1917","position":38,"is_corresponding":false},{"id":1326238,"name":"Guillermo A Gonzalez","orcid":null,"position":39,"is_corresponding":false},{"id":991908,"name":"Karen L Hibbard","orcid":"0000-0002-2001-6099","position":40,"is_corresponding":false},{"id":1326239,"name":"Cuizhen Huang","orcid":null,"position":41,"is_corresponding":false},{"id":1326240,"name":"Jui‐Chun Kao","orcid":null,"position":42,"is_corresponding":false},{"id":603808,"name":"Todd Laverty","orcid":null,"position":43,"is_corresponding":false},{"id":1326241,"name":"Monti Mercer","orcid":null,"position":44,"is_corresponding":false},{"id":1325600,"name":"Brenda Perez","orcid":"0000-0002-2420-402X","position":45,"is_corresponding":false},{"id":1326242,"name":"Scarlett Pitts","orcid":null,"position":46,"is_corresponding":false},{"id":1326243,"name":"Danielle Ruiz","orcid":null,"position":47,"is_corresponding":false},{"id":1326244,"name":"Viruthika Vallanadu","orcid":null,"position":48,"is_corresponding":false},{"id":1326245,"name":"Grace Zhiyu Zheng","orcid":null,"position":49,"is_corresponding":false},{"id":553794,"name":"Cristian Goina","orcid":"0000-0003-2835-7602","position":50,"is_corresponding":false},{"id":553795,"name":"Hideo Otsuna","orcid":"0000-0002-2107-8881","position":51,"is_corresponding":false},{"id":553796,"name":"Konrad Rokicki","orcid":"0000-0002-2799-9833","position":52,"is_corresponding":false},{"id":553797,"name":"Robert Svirskas","orcid":"0000-0001-8374-6008","position":53,"is_corresponding":false},{"id":450862,"name":"Han SJ Cheong","orcid":"0000-0001-5233-3438","position":54,"is_corresponding":false},{"id":984775,"name":"Michael-John Dolan","orcid":"0000-0001-9666-3682","position":55,"is_corresponding":false},{"id":553800,"name":"Erica Ehrhardt","orcid":"0000-0002-9252-1414","position":56,"is_corresponding":false},{"id":281562,"name":"Kai Feng","orcid":"0000-0002-5506-6889","position":57,"is_corresponding":false},{"id":1326246,"name":"Basel EI Galfi","orcid":null,"position":58,"is_corresponding":false},{"id":553801,"name":"Jens Goldammer","orcid":"0000-0002-5623-8339","position":59,"is_corresponding":false},{"id":1325601,"name":"Stephen J Huston","orcid":"0000-0002-0834-3257","position":60,"is_corresponding":false},{"id":311501,"name":"Nan Hu","orcid":"0000-0003-4866-4263","position":61,"is_corresponding":false},{"id":553802,"name":"Masayoshi Ito","orcid":"0000-0002-5624-5918","position":62,"is_corresponding":false},{"id":269906,"name":"Claire McKellar","orcid":"0000-0002-3580-7336","position":63,"is_corresponding":false},{"id":553806,"name":"Ryo Minegishi","orcid":"0000-0002-2895-9438","position":64,"is_corresponding":false},{"id":553807,"name":"Shigehiro Namiki","orcid":"0000-0003-1559-799X","position":65,"is_corresponding":false},{"id":228485,"name":"Aljoscha Nern","orcid":"0000-0002-3822-489X","position":66,"is_corresponding":false},{"id":1166056,"name":"Catherine E. Schretter","orcid":"0000-0002-3957-6838","position":67,"is_corresponding":false},{"id":553808,"name":"Gabriella R Sterne","orcid":"0000-0002-7221-648X","position":68,"is_corresponding":false},{"id":285976,"name":"Lalanti Venkatasubramanian","orcid":"0000-0002-9280-8335","position":69,"is_corresponding":false},{"id":1325602,"name":"Kaiyu Wang","orcid":"0000-0002-6580-9009","position":70,"is_corresponding":false},{"id":553809,"name":"Tanya Wolff","orcid":"0000-0002-8681-1749","position":71,"is_corresponding":false},{"id":1325603,"name":"Ming Wu","orcid":"0000-0002-2193-8271","position":72,"is_corresponding":false},{"id":1326247,"name":"Reed George","orcid":null,"position":73,"is_corresponding":false},{"id":553810,"name":"Oz Malkesman","orcid":"0000-0003-2219-7476","position":74,"is_corresponding":false},{"id":228494,"name":"Yoshinori Aso","orcid":"0000-0002-2939-1688","position":75,"is_corresponding":false},{"id":553798,"name":"Gwyneth M Card","orcid":"0000-0002-7679-3639","position":76,"is_corresponding":false},{"id":553799,"name":"Barry J. Dickson","orcid":"0000-0003-0715-892X","position":77,"is_corresponding":false},{"id":553804,"name":"Wyatt Korff","orcid":"0000-0001-8396-1533","position":78,"is_corresponding":false},{"id":989035,"name":"Kei Ito","orcid":"0000-0002-7274-5533","position":79,"is_corresponding":false},{"id":296061,"name":"James W. Truman","orcid":"0000-0002-9209-5435","position":80,"is_corresponding":false},{"id":883657,"name":"Marta Zlatic","orcid":"0000-0002-3149-2250","position":81,"is_corresponding":false},{"id":65712,"name":"Gerald M. Rubin","orcid":"0000-0001-8762-8703","position":82,"is_corresponding":false},{"id":554938,"name":"FlyLight Project Team","orcid":null,"position":83,"is_corresponding":false},{"id":553787,"name":"Geoffrey W Meissner","orcid":"0000-0003-0369-9788","position":0,"is_corresponding":true}],"reference_count":90,"raw_metadata":null,"created_at":"2026-07-19T02:07:38.055693Z","pmid":"39854223","pmcid":"PMC11759409","fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":55.5556,"fair_a":62.5,"fair_i":20.0,"fair_r":66.6667,"fair_zscore":0.7804,"fair_rationale":{"fair_score":54.17,"has_llm":true,"taxonomy_version":"fair_taxonomy_v5","dimensions":{"F":{"name":"Findable","score":55.56,"criteria":[{"key":"f_dataset_pid","label":"Persistent identifier for the data","kind":"llm","weight":2.0,"fraction":0.5,"verdict":"partial","evidence":"https://doi.org/10.25378/janelia.21266625.v1","grounded":false,"rationale":"The paper provides a DOI for the dataset, which is a persistent identifier scheme. [downgraded to 'partial' — no verifiable quote from the paper] [majority verdict 'partial' (3/5 passes agreed)]","anchors":["RDA-F1-01D — FAIR Data Maturity Model: 'Data is identified by a persistent identifier' (priorit","RDA-F1-02D — FAIR Data Maturity Model: 'Data is identified by a globally unique identifier'","FsF-F1-02D — F-UJI/FAIRsFAIR: 'Data is assigned a persistent identifier'"],"scored":true,"signal":null},{"key":"f_repository_named","label":"Named repository","kind":"llm","weight":2.0,"fraction":1.0,"verdict":"yes","evidence":"we have made all the primary adult data (and a variety of processed outputs) used in this study freely available under a CC BY 4.0 license at https://doi.org/10 .25378/janelia.21266625.v1","grounded":true,"rationale":"The data repository is Janelia (implicit in the DOI) and the Bloomington Drosophila Stock Center is also named; both are recognised repositories. [majority verdict 'yes' (4/5 passes agreed)]","anchors":["RDA-F4-01M — FAIR Data Maturity Model: metadata is offered so it can be harvested and indexed (","NIH DMS Policy Element 4 (NOT-OD-21-014) — name the repository where data will be archived","NSTC Desirable Characteristics of Data Repositories (2022) — 'Long-Term Sustainability', 'Reten"],"scored":true,"signal":null},{"key":"f_data_availability_statement","label":"Data-availability statement","kind":"llm","weight":2.0,"fraction":0.5,"verdict":"partial","evidence":"we have made all the primary adult data (and a variety of processed outputs) used in this study freely available under a CC BY 4.0 license at https://doi.org/10.25378/janelia.21266625.v1 and through the publicly accessible websites https://splitgal4.janelia.org and https://flylight-raw.janelia.org","grounded":false,"rationale":"The statement points to a repository record via a DOI, matching Colavizza category 3. [downgraded to 'partial' — no verifiable quote from the paper] [majority verdict 'partial' (3/5 passes agreed)]","anchors":["Colavizza, Hrynaszkiewicz, Staden, Whitaker & McGillivray (2020), 'The citation advantage of li","Springer Nature research data policy — Data Availability Statements: standard statement templat","RDA-F3-01M — metadata clearly and explicitly includes the identifier of the data it describes"],"scored":false,"signal":null},{"key":"f_discovery_metadata","label":"Description of the dataset as an object","kind":"llm","weight":2.0,"fraction":0.5,"verdict":"partial","evidence":"In total the raw image collection consists of 46,653 IS/SS combinations, 129,665 samples (flies), 612,124 3D image stacks, and 4,371,364 secondary (processed) image outputs, together 192 TB in size.","grounded":true,"rationale":"The dataset's content and size are described in a running prose sentence, not as an itemised section or table. [majority verdict 'partial' (3/5 passes agreed)]","anchors":["RDA-F2-01M — 'Rich metadata is provided to allow discovery' (priority Essential)","FsF-F2-01M — F-UJI: 'Metadata includes descriptive core elements to support data findability'","FsF-R1-01MD — F-UJI: 'Metadata specifies the content of the data'"],"scored":false,"signal":null},{"key":"f_dataset_cited","label":"Dataset formally cited","kind":"llm","weight":1.0,"fraction":0.0,"verdict":"no","evidence":"https://doi.org/10.25378/janelia.21266625.v1","grounded":false,"rationale":"The dataset identifier appears only in the body text (Data availability section), not as a reference-list entry. 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[majority verdict 'yes' (4/5 passes agreed)]","anchors":["FsF-A1-01M — F-UJI: 'Metadata contains access level and access conditions of the data'","RDA-A1-01M — metadata contains information to enable the user to get access to the data","COAR Controlled Vocabularies — Access Rights v1.0 (open / embargoed / restricted / metadata-onl"],"scored":false,"signal":null},{"key":"a_controlled_access_for_sensitive","label":"Gatekeeper for sensitive data","kind":"llm","weight":0.5,"fraction":0.0,"verdict":"no","evidence":null,"grounded":false,"rationale":"The data are not sensitive (fly images and stocks); no gatekeeper is mentioned, so the verdict is 'no'.","anchors":["NIH Genomic Data Sharing Policy (NOT-OD-14-124) — controlled-access via a Data Access Committee","RDA-A1.2-01D — 'Data is accessible through an access protocol that supports authentication and ","NIH DMS Policy Element 5 (NOT-OD-21-014) — Access, Distribution, or Reuse Considerations (conse"],"scored":false,"signal":null},{"key":"a_timeline_retention","label":"Availability timing & retention","kind":"llm","weight":0.5,"fraction":1.0,"verdict":"yes","evidence":"The collection of lines described above has been deposited with Bloomington Drosophila Stock Center for availability until at least August 31, 2026.","grounded":true,"rationale":"The paper makes a specific persistence commitment for the fly stocks, stating they will be available until a specific date. [majority verdict 'yes' (4/5 passes agreed)]","anchors":["NIH DMS Plan Element 4 (NOT-OD-21-014) — Data Preservation, Access, and Associated Timelines","NSTC Desirable Characteristics (2022), Organizational Infrastructure: 'Retention Policy'","RDA-A2-01M — 'Metadata is guaranteed to remain available after data is no longer available'"],"scored":false,"signal":null}]},"I":{"name":"Interoperable","score":20.0,"criteria":[{"key":"i_open_nonproprietary_format","label":"Open file format","kind":"llm","weight":1.0,"fraction":0.0,"verdict":"no","evidence":null,"grounded":false,"rationale":"No specific file format token (e.g., TIFF, PNG) is named for the released data; only '3D image stacks' is mentioned.","anchors":["FsF-R1.3-02D — F-UJI: 'Data is available in a file format recommended by the target research co","RDA-R1.3-02D — data is expressed in a machine-understandable community standard","RDA-I1-01D — data uses a knowledge representation expressed in a standardised format"],"scored":true,"signal":null},{"key":"i_community_standard_vocabulary","label":"Community standard / vocabulary","kind":"llm","weight":1.0,"fraction":0.0,"verdict":"no","evidence":null,"grounded":false,"rationale":"No named data or metadata community standard from FAIRsharing is mentioned; only generic 'standard protocols'.","anchors":["RDA-R1.3-01M — 'Metadata complies with a community standard' (priority Essential)","RDA-R1.3-01D — 'Data complies with a community standard'","RDA-I2-01M — '(Meta)data use vocabularies that follow FAIR principles'"],"scored":false,"signal":null},{"key":"i_qualified_references","label":"Identifiers for the resources the data depend on","kind":"llm","weight":0.5,"fraction":1.0,"verdict":"yes","evidence":"https://doi.org/10.1371/journal.pone.0236495","grounded":true,"rationale":"The paper includes DOIs for external resources, such as the JRC2018 template, in the reference list. [majority verdict 'yes' (3/5 passes agreed)]","anchors":["RDA-I3-01M — '(meta)data include references to other (meta)data'","RDA-I3-03M — 'metadata includes qualified references to other metadata'","FsF-I3-01M — F-UJI: 'Metadata includes links between the data and its related entities'"],"scored":false,"signal":null}]},"R":{"name":"Reusable","score":66.67,"criteria":[{"key":"r_reuse_license","label":"Reuse licence","kind":"llm","weight":2.0,"fraction":1.0,"verdict":"yes","evidence":"freely available under a CC BY 4.0 license","grounded":true,"rationale":"The data are licensed under CC BY 4.0, which is an open standard license. 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[majority verdict 'yes' (3/5 passes agreed)]","anchors":["RDA-R1.2-01M — 'Metadata includes provenance information according to community- specific standa","FsF-R1.2-01M — F-UJI: 'Metadata includes provenance information about data creation or generati","W3C PROV-O (W3C Recommendation, 2013) — the entity/activity/agent model of provenance"],"scored":false,"signal":null},{"key":"r_documentation_codebook","label":"Documentation / codebook","kind":"llm","weight":1.0,"fraction":0.5,"verdict":"partial","evidence":"Figure 1 Supplemental file 1. Spreadsheet of cell-type-specific adult and larval split-GAL4 lines.","grounded":true,"rationale":"Variable definitions (spreadsheet) are provided as a supplementary file inside the article, not as a separate documentation file shipped with the data. [majority verdict 'partial' (4/5 passes agreed)]","anchors":["RDA-R1-01M — '(Meta)data are richly described with a plurality of accurate and relevant attribu","FsF-R1-01MD — F-UJI: 'Metadata specifies the content of the data'","NIH DMS Policy Element 3 (NOT-OD-21-014) — Standards (documentation and metadata to accompany t"],"scored":false,"signal":null},{"key":"r_versioning","label":"Snapshot identified","kind":"llm","weight":0.5,"fraction":0.5,"verdict":"partial","evidence":"10.25378/janelia.21266625.v1","grounded":false,"rationale":"The DOI includes a version suffix 'v1', indicating a specific snapshot of the data. [downgraded to 'partial' — no verifiable quote from the paper] [majority verdict 'partial' (4/5 passes agreed)]","anchors":["DataCite Metadata Schema 4.6 — the 'Version' property","RDA-R1.2-01M — provenance information (which version was used is provenance)","NSTC Desirable Characteristics of Data Repositories (2022) — 'Provenance', 'Retention Policy'"],"scored":true,"signal":null},{"key":"x_code_availability","label":"Analysis code available","kind":"llm","weight":1.0,"fraction":0.0,"verdict":"no","evidence":"Additional details of image processing, including source code for processing scripts, are available at https://data.janelia.org/pipeline.","grounded":false,"rationale":"The code is at a project URL, not a versioned archive or code-forge repository. [downgraded to 'no' — no verifiable quote from the paper] [majority verdict 'no' (2/5 passes agreed)]","anchors":["NIH DMS Policy Element 2 (NOT-OD-21-014) — 'Related Tools, Software and/or Code'","FAIR4RS Principles v1.0 (Chue Hong et al., 2022; RDA/FORCE11/ReSA) — FAIR Principles for Resear","FORCE11 Software Citation Principles (Smith, Katz & Niemeyer, 2016, PeerJ CS 2:e86)"],"scored":true,"signal":null},{"key":"x_funding_attribution","label":"Funder and award number","kind":"llm","weight":0.5,"fraction":0.5,"verdict":"partial","evidence":"Funding was provided by Howard Hughes Medical Institute.","grounded":true,"rationale":"The funder is named (Howard Hughes Medical Institute) but no award/grant number is provided. [majority verdict 'partial' (4/5 passes agreed)]","anchors":["DataCite Metadata Schema 4.6 — 'FundingReference' property (funderName, funderIdentifier, award","Crossref Funder Registry — canonical funder identifiers for funding metadata","RDA-F2-01M — rich metadata provided to allow discovery (funding is part of the descriptive reco"],"scored":true,"signal":null}]}},"actions":[{"key":"f_dataset_pid","dimension":"F","label":"Persistent identifier for the data","action":"Mint or cite a persistent identifier for the dataset — a repository DOI or an accession from a registered repository — and print it in the paper. A bare URL is not persistent: it is the single most common cause of a dead data link five years after publication. For proteomics data, deposit in PRIDE (PXD accession) or ProteomeXchange.","anchors":["yes","partial","no"],"verdict":"partial","current":0.5,"evidence":"https://doi.org/10.25378/janelia.21266625.v1","why":"The paper provides a DOI for the dataset, which is a persistent identifier scheme. [downgraded to 'partial' — no verifiable quote from the paper] [majority verdict 'partial' (3/5 passes agreed)]","gain":8.33,"priority":"essential","scored":true},{"key":"a_data_openly_accessible","dimension":"A","label":"Access route free of preconditions","action":"Remove the precondition or justify it. Release the data at publication with no embargo, no registration wall, and no approval step — NIH's zero-embargo public- access rule (NOT-OD-25-101) has already made 'available at publication' the federal baseline for the article; the data should not lag behind it. For proteomics data, deposit in PRIDE (PXD accession) or ProteomeXchange.","anchors":["yes","partial","no"],"verdict":"partial","current":0.5,"evidence":"we have made all the primary adult data (and a variety of processed outputs) used in this study freely available under a CC BY 4.0 license at https://doi.org/10.25378/janelia.21266625.v1","why":"The data are stated to be freely available with no precondition, via a DOI link. [downgraded to 'partial' — no verifiable quote from the paper] [majority verdict 'partial' (3/5 passes agreed)]","gain":8.33,"priority":"essential","scored":true},{"key":"f_dataset_cited","dimension":"F","label":"Dataset formally cited","action":"Cite the dataset in the reference list like a publication — creator, year, title, repository, DOI/accession — and cite it in-text where it is used. Only a reference- list entry is machine-readable to Crossref/DataCite, and only a citation lets the data earn credit. Cite the proteomics repository accession (e.g. from PRIDE (PXD accession) or ProteomeXchange) in the reference list.","anchors":["yes","partial","no"],"verdict":"no","current":0.0,"evidence":"https://doi.org/10.25378/janelia.21266625.v1","why":"The dataset identifier appears only in the body text (Data availability section), not as a reference-list entry. [downgraded to 'no' — no verifiable quote from the paper] [majority verdict 'no' (4/5 passes agreed)]","gain":8.33,"priority":"important","scored":true},{"key":"i_open_nonproprietary_format","dimension":"I","label":"Open file format","action":"Release the data in an open, community-standard format (CSV/TSV, JSON, HDF5, NetCDF, FASTQ, VCF, NIfTI…) instead of — or alongside — any proprietary or instrument-native format, and name the format in the paper. A dataset that needs a €2,000 licence to open is not reusable. Prefer open proteomics formats such as mzML or mzIdentML.","anchors":["yes","partial","no"],"verdict":"no","current":0.0,"evidence":null,"why":"No specific file format token (e.g., TIFF, PNG) is named for the released data; only '3D image stacks' is mentioned.","gain":8.33,"priority":"important","scored":true},{"key":"x_code_availability","dimension":"R","label":"Analysis code available","action":"Publish the analysis code in a public forge, archive a tagged release with a DOI (Zenodo/Software Heritage), and cite that DOI in the paper. NIH DMS Element 2 asks for the tools and code, not only the data — and 'available on request' is not a locator. Archive the analysis code in a versioned repository (GitHub + a Zenodo release DOI).","anchors":["yes","partial","no"],"verdict":"no","current":0.0,"evidence":"Additional details of image processing, including source code for processing scripts, are available at https://data.janelia.org/pipeline.","why":"The code is at a project URL, not a versioned archive or code-forge repository. [downgraded to 'no' — no verifiable quote from the paper] [majority verdict 'no' (2/5 passes agreed)]","gain":8.33,"priority":"important","scored":true},{"key":"r_versioning","dimension":"R","label":"Snapshot identified","action":"Version the deposit and cite the exact version analysed (a version-specific DOI, or an accession with its version suffix). A reader reproducing your work against 'the current release' is reproducing it against a different dataset.","anchors":["yes","partial","no"],"verdict":"partial","current":0.5,"evidence":"10.25378/janelia.21266625.v1","why":"The DOI includes a version suffix 'v1', indicating a specific snapshot of the data. [downgraded to 'partial' — no verifiable quote from the paper] [majority verdict 'partial' (4/5 passes agreed)]","gain":2.08,"priority":"useful","scored":true},{"key":"x_funding_attribution","dimension":"R","label":"Funder and award number","action":"State the funder AND the award number in the paper, and put them in the dataset's FundingReference metadata. A funder name alone cannot be linked back to the award, so the funding provenance of the data is lost the moment the paper is indexed.","anchors":["yes","partial","no"],"verdict":"partial","current":0.5,"evidence":"Funding was provided by Howard Hughes Medical Institute.","why":"The funder is named (Howard Hughes Medical Institute) but no award/grant number is provided. [majority verdict 'partial' (4/5 passes agreed)]","gain":2.08,"priority":"useful","scored":true},{"key":"f_data_availability_statement","dimension":"F","label":"Data-availability statement","action":"Replace the statement with the repository template: name the repository and give the accession or DOI (Colavizza category 3). This is the only DAS class associated with a measured citation advantage; 'available on reasonable request' and 'within the article' are not.","anchors":["yes","partial","no"],"verdict":"partial","current":0.5,"evidence":"we have made all the primary adult data (and a variety of processed outputs) used in this study freely available under a CC BY 4.0 license at https://doi.org/10.25378/janelia.21266625.v1 and through the publicly accessible websites https://splitgal4.janelia.org and https://flylight-raw.janelia.org","why":"The statement points to a repository record via a DOI, matching Colavizza category 3. [downgraded to 'partial' — no verifiable quote from the paper] [majority verdict 'partial' (3/5 passes agreed)]","gain":0.0,"priority":"essential","scored":false},{"key":"f_discovery_metadata","dimension":"F","label":"Description of the dataset as an object","action":"Add a 'Data Records' section: itemise every file in the deposit and every variable or sample it holds, with counts and units. Describe the dataset as an object in its own right, not as a by-product of the findings — this is what makes it discoverable to someone who is not looking for your paper.","anchors":["yes","partial","no"],"verdict":"partial","current":0.5,"evidence":"In total the raw image collection consists of 46,653 IS/SS combinations, 129,665 samples (flies), 612,124 3D image stacks, and 4,371,364 secondary (processed) image outputs, together 192 TB in size.","why":"The dataset's content and size are described in a running prose sentence, not as an itemised section or table. [majority verdict 'partial' (3/5 passes agreed)]","gain":0.0,"priority":"essential","scored":false},{"key":"i_community_standard_vocabulary","dimension":"I","label":"Community standard / vocabulary","action":"Adopt and NAME your domain's data standard — the minimum-information checklist, metadata schema, or ontology your community uses (MIAME/MINSEQE, ISA-Tab, BIDS, an OBO ontology, HL7 FHIR/OMOP) — and say which one you followed. A reporting checklist standardises your paper; it does nothing for your data. In proteomics, describe the data with mzML or MIAPE.","anchors":["yes","partial","no"],"verdict":"no","current":0.0,"evidence":null,"why":"No named data or metadata community standard from FAIRsharing is mentioned; only generic 'standard protocols'.","gain":0.0,"priority":"important","scored":false},{"key":"r_documentation_codebook","dimension":"R","label":"Documentation / codebook","action":"Ship a README and a data dictionary IN the deposit — every file, every variable, its units, its allowed values, its missing-value codes. It is the cheapest single thing that makes a dataset usable by someone who was not in the lab, and a table buried in the article does not travel with the data.","anchors":["yes","partial","no"],"verdict":"partial","current":0.5,"evidence":"Figure 1 Supplemental file 1. Spreadsheet of cell-type-specific adult and larval split-GAL4 lines.","why":"Variable definitions (spreadsheet) are provided as a supplementary file inside the article, not as a separate documentation file shipped with the data. [majority verdict 'partial' (4/5 passes agreed)]","gain":0.0,"priority":"important","scored":false},{"key":"a_controlled_access_for_sensitive","dimension":"A","label":"Gatekeeper for sensitive data","action":"Route sensitive data through an institutional gatekeeper — deposit in a controlled- access repository (dbGaP, EGA) with a Data Access Committee and a published DUA — rather than through the corresponding author's inbox. An author-gated dataset dies with the author's email address, and 'on reasonable request' has been shown repeatedly not to yield data.","anchors":["yes","partial","no"],"verdict":"no","current":0.0,"evidence":null,"why":"The data are not sensitive (fly images and stocks); no gatekeeper is mentioned, so the verdict is 'no'.","gain":0.0,"priority":"useful","scored":false}],"suggestions":["Mint or cite a persistent identifier for the dataset — a repository DOI or an accession from a registered repository — and print it in the paper. A bare URL is not persistent: it is the single most common cause of a dead data link five years after publication. For proteomics data, deposit in PRIDE (PXD accession) or ProteomeXchange.","Remove the precondition or justify it. Release the data at publication with no embargo, no registration wall, and no approval step — NIH's zero-embargo public- access rule (NOT-OD-25-101) has already made 'available at publication' the federal baseline for the article; the data should not lag behind it. For proteomics data, deposit in PRIDE (PXD accession) or ProteomeXchange.","Cite the dataset in the reference list like a publication — creator, year, title, repository, DOI/accession — and cite it in-text where it is used. Only a reference- list entry is machine-readable to Crossref/DataCite, and only a citation lets the data earn credit. Cite the proteomics repository accession (e.g. from PRIDE (PXD accession) or ProteomeXchange) in the reference list.","Release the data in an open, community-standard format (CSV/TSV, JSON, HDF5, NetCDF, FASTQ, VCF, NIfTI…) instead of — or alongside — any proprietary or instrument-native format, and name the format in the paper. A dataset that needs a €2,000 licence to open is not reusable. Prefer open proteomics formats such as mzML or mzIdentML.","Publish the analysis code in a public forge, archive a tagged release with a DOI (Zenodo/Software Heritage), and cite that DOI in the paper. NIH DMS Element 2 asks for the tools and code, not only the data — and 'available on request' is not a locator. Archive the analysis code in a versioned repository (GitHub + a Zenodo release DOI)."],"model":"deepseek/deepseek-v4-flash","agent_version":"fair_agent_v8","fulltext_source":"unpaywall_pdf"},"fair_model":"deepseek/deepseek-v4-flash","fair_agent_version":"fair_agent_v8","fair_fulltext_source":"unpaywall_pdf","fair_has_llm":true,"fair_computed_at":"2026-07-20T11:55:43.920649Z","clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}