{"doi":"10.7554/elife.95528","title":"High-resolution awake mouse fMRI at 14 tesla","abstract":"High-resolution awake mouse functional magnetic resonance imaging (fMRI) remains challenging despite extensive efforts to address motion-induced artifacts and stress. This study introduces an implantable radio frequency (RF) surface coil design that minimizes image distortion caused by the air/tissue interface of mouse brains while simultaneously serving as a headpost for fixation during scanning. Furthermore, this study provides a thorough acclimation method used to accustom animals to the MRI environment minimizing motion-induced artifacts. Using a 14 T scanner, high-resolution fMRI enabled brain-wide functional mapping of visual and vibrissa stimulation at 100 µm×100 µm×200 µm resolution with a 2 s per frame sampling rate. Besides activated ascending visual and vibrissa pathways, robust blood oxygen level-dependent (BOLD) responses were detected in the anterior cingulate cortex upon visual stimulation and spread through the ventral retrosplenial area (VRA) with vibrissa air-puff stimulation, demonstrating higher-order sensory processing in association cortices of awake mice. In particular, the rapid hemodynamic responses in VRA upon vibrissa stimulation showed a strong correlation with the hippocampus, thalamus, and prefrontal cortical areas. Cross-correlation analysis with designated VRA responses revealed early positive BOLD signals at the contralateral barrel cortex (BC) occurring 2 s prior to the air-puff in awake mice with repetitive stimulation, which was not detected using a randomized stimulation paradigm. This early BC activation indicated a learned anticipation through the vibrissa system and association cortices in awake mice under continuous exposure of repetitive air-puff stimulation. This work establishes a high-resolution awake mouse fMRI platform, enabling brain-wide functional mapping of sensory signal processing in higher association cortical areas.","journal":"eLife","year":2024,"id":484265,"datarank":0.40648382874459826,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"self_citation_contribution":0.38474240361923057,"citation_network_contribution":0.02174142512536772,"self_endowment_contribution":0.38474240361923057,"citer_contribution":0.02174142512536772,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":5,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9521,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1170748,"name":"Xiaochen Liu","orcid":"0000-0001-6342-7704","position":1,"is_corresponding":false},{"id":1170749,"name":"Zeping Xie","orcid":"0000-0002-3120-5565","position":2,"is_corresponding":false},{"id":1020868,"name":"Bei Zhang","orcid":"0000-0003-0845-7534","position":3,"is_corresponding":false},{"id":575981,"name":"Sangcheon Choi","orcid":"0000-0001-7327-1344","position":4,"is_corresponding":false},{"id":930828,"name":"Xiaoqing Zhou","orcid":"0000-0002-4139-032X","position":5,"is_corresponding":false},{"id":336377,"name":"Andy Liu","orcid":"0000-0003-3096-1788","position":6,"is_corresponding":false},{"id":1171090,"name":"Alyssa Murstein","orcid":null,"position":7,"is_corresponding":false},{"id":575980,"name":"Yuanyuan Jiang","orcid":"0000-0001-7758-7450","position":8,"is_corresponding":false},{"id":246429,"name":"Anna Devor","orcid":"0000-0002-5143-3960","position":9,"is_corresponding":false},{"id":251801,"name":"Xin Yu","orcid":"0000-0001-9890-5489","position":10,"is_corresponding":false},{"id":622898,"name":"David Hike","orcid":"0000-0001-5294-1767","position":0,"is_corresponding":true}],"reference_count":151,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:07:38.055693Z","pmid":"39786364","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}