{"doi":"10.7554/elife.94073","title":"Expanding the Drosophila toolkit for dual control of gene expression","abstract":"The ability to independently control gene expression in two different tissues in the same animal is emerging as a major need, especially in the context of inter-organ communication studies. This type of study is made possible by technologies combining the GAL4/UAS and a second binary expression system such as the LexA system or QF system. Here, we describe a resource of reagents that facilitate combined use of the GAL4/UAS and a second binary system in various Drosophila tissues. Focusing on genes with well-characterized GAL4 expression patterns, we generated a set of more than 40 LexA-GAD and QF2 insertions by CRISPR knock-in and verified their tissue specificity in larvae. We also built constructs that encode QF2 and LexA-GAD transcription factors in a single vector. Following successful integration of this construct into the fly genome, FLP/FRT recombination is used to isolate fly lines that express only QF2 or LexA-GAD. Finally, using new compatible shRNA vectors, we evaluated both LexA and QF systems for in vivo gene knockdown and are generating a library of such RNAi fly lines as a community resource. Together, these LexA and QF system vectors and fly lines will provide a new set of tools for researchers who need to activate or repress two different genes in an orthogonal manner in the same animal.","journal":"eLife","year":2023,"id":332983,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":24,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9537,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1060202,"name":"Barbara Jusiak","orcid":null,"position":1,"is_corresponding":false},{"id":1060203,"name":"Raphael Lopes","orcid":null,"position":2,"is_corresponding":false},{"id":259034,"name":"Ben Ewen‐Campen","orcid":"0000-0002-4326-3033","position":3,"is_corresponding":false},{"id":264325,"name":"Justin A. Bosch","orcid":"0000-0001-8499-1566","position":4,"is_corresponding":false},{"id":1060204,"name":"Alexandria Risbeck","orcid":null,"position":5,"is_corresponding":false},{"id":684385,"name":"Corey Forman","orcid":null,"position":6,"is_corresponding":false},{"id":261195,"name":"Christians Villalta","orcid":null,"position":7,"is_corresponding":false},{"id":259031,"name":"Yanhui Hu","orcid":"0000-0003-1494-1402","position":8,"is_corresponding":false},{"id":14957,"name":"Norbert Perrimon","orcid":"0000-0001-7542-472X","position":9,"is_corresponding":false},{"id":259030,"name":"Jonathan Zirin","orcid":"0009-0003-8242-5044","position":0,"is_corresponding":true}],"reference_count":44,"raw_metadata":null,"created_at":"2026-07-19T01:09:35.125543Z","pmid":"38569007","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}