{"doi":"10.7554/elife.91809","title":"Illuminating T cell-dendritic cell interactions in vivo by FlAsHing antigens","abstract":"Delineating the complex network of interactions between antigen-specific T cells and antigen presenting cells (APCs) is crucial for effective precision therapies against cancer, chronic infections, and autoimmunity. However, the existing arsenal for examining antigen-specific T cell interactions is restricted to a select few antigen-T cell receptor pairs, with limited in situ utility. This lack of versatility is largely due to the disruptive effects of reagents on the immune synapse, which hinder real-time monitoring of antigen-specific interactions. To address this limitation, we have developed a novel and versatile immune monitoring strategy by adding a short cysteine-rich tag to antigenic peptides that emits fluorescence upon binding to thiol-reactive biarsenical hairpin compounds. Our findings demonstrate the specificity and durability of the novel antigen-targeting probes during dynamic immune monitoring in vitro and in vivo. This strategy opens new avenues for biological validation of T-cell receptors with newly identified epitopes by revealing the behavior of previously unrecognized antigen-receptor pairs, expanding our understanding of T cell responses.","journal":"eLife","year":2023,"id":385568,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9549,"is_data_producer":true,"deposit_databanks":{"Dryad":["10.5061/dryad.v15dv4239"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1153576,"name":"Jafar Al Souz","orcid":"0000-0003-2345-2803","position":1,"is_corresponding":false},{"id":348593,"name":"Daniel Williams","orcid":"0000-0001-8512-0359","position":2,"is_corresponding":false},{"id":892559,"name":"Rahul Kamdar","orcid":null,"position":3,"is_corresponding":false},{"id":287787,"name":"Olena Kamenyeva","orcid":"0000-0002-6541-5616","position":4,"is_corresponding":false},{"id":243363,"name":"Juraj Kabát","orcid":"0000-0001-8636-542X","position":5,"is_corresponding":false},{"id":321383,"name":"Ethan M. Shevach","orcid":"0000-0003-1607-4664","position":6,"is_corresponding":false},{"id":353206,"name":"Billur Akkaya","orcid":"0000-0002-6808-3776","position":7,"is_corresponding":false},{"id":420584,"name":"Munir Akkaya","orcid":"0000-0002-9949-9424","position":0,"is_corresponding":true}],"reference_count":37,"raw_metadata":null,"created_at":"2026-07-19T01:17:52.636565Z","pmid":"38236633","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}