{"doi":"10.7554/elife.90116","title":"Inhibition of Cpeb3 ribozyme elevates CPEB3 protein expression and polyadenylation of its target mRNAs and enhances object location memory","abstract":"A self-cleaving ribozyme that maps to an intron of the cytoplasmic polyadenylation element-binding protein 3 ( Cpeb3 ) gene is thought to play a role in human episodic memory, but the underlying mechanisms mediating this effect are not known. We tested the activity of the murine sequence and found that the ribozyme’s self-scission half-life matches the time it takes an RNA polymerase to reach the immediate downstream exon, suggesting that the ribozyme-dependent intron cleavage is tuned to co-transcriptional splicing of the Cpeb3 mRNA. Our studies also reveal that the murine ribozyme modulates maturation of its harboring mRNA in both cultured cortical neurons and the hippocampus: inhibition of the ribozyme using an antisense oligonucleotide leads to increased CPEB3 protein expression, which enhances polyadenylation and translation of localized plasticity-related target mRNAs, and subsequently strengthens hippocampal-dependent long-term memory. These findings reveal a previously unknown role for self-cleaving ribozyme activity in regulating experience-induced co-transcriptional and local translational processes required for learning and memory.","journal":"eLife","year":2024,"id":453310,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9571,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":340922,"name":"Joseph Han","orcid":null,"position":1,"is_corresponding":false},{"id":740451,"name":"Carlene A. Chinn","orcid":null,"position":2,"is_corresponding":false},{"id":1169581,"name":"J. Rounds","orcid":null,"position":3,"is_corresponding":false},{"id":1275854,"name":"Xiang Li","orcid":"0000-0002-3133-7365","position":4,"is_corresponding":false},{"id":810686,"name":"Mehran Nikan","orcid":null,"position":5,"is_corresponding":false},{"id":810687,"name":"Marie Myszka","orcid":null,"position":6,"is_corresponding":false},{"id":810059,"name":"Liqi Tong","orcid":"0000-0001-9470-5885","position":7,"is_corresponding":false},{"id":496034,"name":"Luiz F. M. Passalacqua","orcid":"0000-0002-5490-2427","position":8,"is_corresponding":false},{"id":318747,"name":"Timothy W. Bredy","orcid":"0000-0003-3280-126X","position":9,"is_corresponding":false},{"id":509552,"name":"Marcelo A. Wood","orcid":"0000-0003-2029-4176","position":10,"is_corresponding":false},{"id":496038,"name":"Andrej Lupták","orcid":"0000-0002-0632-5442","position":11,"is_corresponding":false},{"id":810058,"name":"Claire C. Chen","orcid":"0000-0003-2490-2909","position":0,"is_corresponding":true}],"reference_count":87,"raw_metadata":null,"created_at":"2026-07-19T02:02:58.784290Z","pmid":"38319152","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}