{"doi":"10.7554/elife.89903","title":"Essential function of transmembrane transcription factor MYRF in promoting transcription of miRNA lin-4 during C. elegans development","abstract":"Precise developmental timing control is essential for organism formation and function, but its mechanisms are unclear. In C. elegans , the microRNA lin-4 critically regulates developmental timing by post-transcriptionally downregulating the larval-stage-fate controller LIN-14. However, the mechanisms triggering the activation of lin-4 expression toward the end of the first larval stage remain unknown. We demonstrate that the transmembrane transcription factor MYRF-1 is necessary for lin-4 activation. MYRF-1 is initially localized on the cell membrane, and its increased cleavage and nuclear accumulation coincide with lin-4 expression timing. MYRF-1 regulates lin-4 expression cell-autonomously and hyperactive MYRF-1 can prematurely drive lin-4 expression in embryos and young first-stage larvae. The tandem lin-4 promoter DNA recruits MYRF-1 GFP to form visible loci in the nucleus, suggesting that MYRF-1 directly binds to the lin-4 promoter. Our findings identify a crucial link in understanding developmental timing regulation and establish MYRF-1 as a key regulator of lin-4 expression.","journal":"eLife","year":2023,"id":330675,"datarank":0.3827825874352874,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.023098296515531764,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.023098296515531764,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":5,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9488,"is_data_producer":true,"deposit_databanks":{"Dryad":["10.5061/dryad.tqjq2bw71"],"GEO":["GSE262766"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":130567,"name":"Zhao Wang","orcid":"0000-0003-0418-6845","position":1,"is_corresponding":false},{"id":1054941,"name":"Lifang Wang","orcid":"0000-0002-6235-3069","position":2,"is_corresponding":false},{"id":724060,"name":"Yingchuan Qi","orcid":"0000-0002-4267-4770","position":3,"is_corresponding":false},{"id":1054940,"name":"Zhimin Xu","orcid":"0009-0007-6371-9345","position":0,"is_corresponding":true}],"reference_count":56,"raw_metadata":null,"created_at":"2026-07-19T01:09:14.691473Z","pmid":"38963411","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}