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Transcriptomic profiling of macrophages showed a highly dynamic signature during infection characterized by a switch from pro-inflammatory to anti-inflammatory/pro-regenerative status and revealed a shift in adhesion program. In agreement with this specific adhesion signature, macrophage trajectory tracking identifies motionless macrophages as a permissive niche for persistent\n                    <jats:italic>Salmonella</jats:italic>\n                    . Our results demonstrate that zebrafish model provides a unique platform to explore, in a whole organism, the versatile nature of macrophage functional programs during bacterial acute and persistent infections.\n                  </jats:p>","journal":"eLife","year":2024,"id":661557,"datarank":0.4566783656585135,"base_score":3.044522437723423,"endowment":3.044522437723423,"self_citation_contribution":0.4566783656585135,"citation_network_contribution":0.0,"self_endowment_contribution":0.4566783656585135,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1727070,"name":"Tamara Sipka","orcid":null,"position":1,"is_corresponding":false},{"id":1727071,"name":"Christina Begon-Pescia","orcid":null,"position":2,"is_corresponding":false},{"id":1727072,"name":"Matteo Bernardello","orcid":null,"position":3,"is_corresponding":false},{"id":1727074,"name":"Sofiane Tairi","orcid":null,"position":4,"is_corresponding":false},{"id":202949,"name":"Lionello Bossi","orcid":null,"position":5,"is_corresponding":false},{"id":1727076,"name":"Anne-Alicia Gonzalez","orcid":null,"position":6,"is_corresponding":false},{"id":1727077,"name":"Xavier Mialhe","orcid":null,"position":7,"is_corresponding":false},{"id":1727078,"name":"Emilio J Gualda","orcid":null,"position":8,"is_corresponding":false},{"id":1727079,"name":"Pablo Loza-Alvarez","orcid":"0000-0002-3129-1213","position":9,"is_corresponding":false},{"id":1727080,"name":"Anne Blanc-Potard","orcid":null,"position":10,"is_corresponding":false},{"id":25085,"name":"Georges Lutfalla","orcid":"0000-0002-6591-7894","position":11,"is_corresponding":false},{"id":1727081,"name":"Mai E Nguyen-Chi","orcid":"0000-0003-2672-2426","position":12,"is_corresponding":false},{"id":1727069,"name":"Jade Leiba","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Dynamics of macrophage polarization support Salmonella persistence in a whole living organism","abstract":"<jats:p>\n                    Numerous intracellular bacterial pathogens interfere with macrophage function, including macrophage polarization, to establish a niche and persist. However, the spatiotemporal dynamics of macrophage polarization during infection within host remain to be investigated. Here, we implement a model of persistent\n                    <jats:italic>Salmonella</jats:italic>\n                    Typhimurium infection in zebrafish, which allows visualization of polarized macrophages and bacteria in real time at high resolution. While macrophages polarize toward M1-like phenotype to control early infection, during later stages,\n                    <jats:italic>Salmonella</jats:italic>\n                    persists inside non-inflammatory clustered macrophages. Transcriptomic profiling of macrophages showed a highly dynamic signature during infection characterized by a switch from pro-inflammatory to anti-inflammatory/pro-regenerative status and revealed a shift in adhesion program. In agreement with this specific adhesion signature, macrophage trajectory tracking identifies motionless macrophages as a permissive niche for persistent\n                    <jats:italic>Salmonella</jats:italic>\n                    . 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