{"doi":"10.7554/elife.89596","title":"Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in mouse pancreatic β cells","abstract":"In pancreatic islet β cells, molecular motors use cytoskeletal polymers microtubules as tracks for intracellular transport of insulin secretory granules. The β-cell microtubule network has a complex architecture and is nondirectional, which provides insulin granules at the cell periphery for rapid secretion response, yet to avoid oversecretion and subsequent hypoglycemia. We have previously characterized a peripheral sub-membrane microtubule array, which is critical for the withdrawal of excessive insulin granules from the secretion sites. Microtubules in β cells originate at the Golgi in the cell interior, and how the peripheral array is formed remains unknown. Here, we demonstrate that kinesin KIF5B, a motor protein with the capacity to transport microtubules as cargos, is needed to align sub-membrane microtubules in clonal mouse β cells MIN6 and β cells within intact mouse islets. Real-time imaging and photokinetics approaches indicate that KIF5B actively slides existing microtubules to the β-cell periphery. Moreover, like many physiological β-cell features, microtubule sliding is facilitated by a high glucose stimulus. These new data, together with our previous report that high glucose destabilizes the sub-membrane microtubule array to allow for robust secretion, indicate that MT sliding is another integral part of glucose-triggered microtubule remodeling, likely replacing destabilized peripheral microtubules to prevent their loss over time and β-cell malfunction.","journal":"eLife","year":2023,"id":397016,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9495,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1173025,"name":"Margret A Fye","orcid":null,"position":1,"is_corresponding":false},{"id":815246,"name":"Alisa Cario","orcid":"0000-0003-3044-0421","position":2,"is_corresponding":false},{"id":699858,"name":"Kung-Hsien Ho","orcid":null,"position":3,"is_corresponding":false},{"id":1173026,"name":"Pi'illani Noguchi","orcid":null,"position":4,"is_corresponding":false},{"id":502802,"name":"Guoqiang Gu","orcid":"0000-0003-0772-9139","position":5,"is_corresponding":false},{"id":397953,"name":"Irina Kaverina","orcid":"0000-0002-4002-8599","position":6,"is_corresponding":false},{"id":910436,"name":"Kai M Bracey","orcid":"0009-0001-5483-0718","position":0,"is_corresponding":true}],"reference_count":44,"raw_metadata":null,"created_at":"2026-07-19T01:19:35.497854Z","pmid":"41182903","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}