{"doi":"10.7554/elife.88328","title":"Comparative interactome analysis of α-arrestin families in human and Drosophila","abstract":"The α-arrestins form a large family of evolutionally conserved modulators that control diverse signaling pathways, including both G-protein-coupled receptor (GPCR)-mediated and non-GPCR-mediated pathways, across eukaryotes. However, unlike β-arrestins, only a few α-arrestin targets and functions have been characterized. Here, using affinity purification and mass spectrometry, we constructed interactomes for 6 human and 12 Drosophila α-arrestins. The resulting high-confidence interactomes comprised 307 and 467 prey proteins in human and Drosophila , respectively. A comparative analysis of these interactomes predicted not only conserved binding partners, such as motor proteins, proteases, ubiquitin ligases, RNA splicing factors, and GTPase-activating proteins, but also those specific to mammals, such as histone modifiers and the subunits of V-type ATPase. Given the manifestation of the interaction between the human α-arrestin, TXNIP, and the histone-modifying enzymes, including HDAC2, we undertook a global analysis of transcription signals and chromatin structures that were affected by TXNIP knockdown. We found that TXNIP activated targets by blocking HDAC2 recruitment to targets, a result that was validated by chromatin immunoprecipitation assays. Additionally, the interactome for an uncharacterized human α-arrestin ARRDC5 uncovered multiple components in the V-type ATPase, which plays a key role in bone resorption by osteoclasts. Our study presents conserved and species-specific protein–protein interaction maps for α-arrestins, which provide a valuable resource for interrogating their cellular functions for both basic and clinical research.","journal":"eLife","year":2023,"id":383531,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8167,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":686102,"name":"Inez K.A. Pranoto","orcid":"0000-0002-0709-5642","position":1,"is_corresponding":false},{"id":1150620,"name":"Soon‐Young Kim","orcid":"0009-0001-5132-7797","position":2,"is_corresponding":false},{"id":1150621,"name":"Hee‐Joo Choi","orcid":"0000-0001-6432-8193","position":3,"is_corresponding":false},{"id":1150622,"name":"Ngoc Bao To","orcid":"0000-0003-3252-4143","position":4,"is_corresponding":false},{"id":1151002,"name":"Hansong Chae","orcid":null,"position":5,"is_corresponding":false},{"id":14386,"name":"Jeong‐Yeon Lee","orcid":"0000-0003-1298-7466","position":6,"is_corresponding":false},{"id":31995,"name":"Jung‐Eun Kim","orcid":"0000-0002-3342-8359","position":7,"is_corresponding":false},{"id":462902,"name":"Young V. Kwon","orcid":"0000-0002-8937-3182","position":8,"is_corresponding":false},{"id":1150623,"name":"Jin‐Wu Nam","orcid":"0000-0003-0047-3687","position":9,"is_corresponding":false},{"id":1150619,"name":"Kyung-Tae Lee","orcid":"0000-0001-5354-6910","position":0,"is_corresponding":true}],"reference_count":121,"raw_metadata":null,"created_at":"2026-07-19T01:17:29.050285Z","pmid":"38270169","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}