{"doi":"10.7554/elife.86204","title":"Regulation of nuclear transcription by mitochondrial RNA in endothelial cells","abstract":"Chromatin-associated RNAs (caRNAs) form a relatively poorly recognized layer of the epigenome. The caRNAs reported to date are transcribed from the nuclear genome. Here, leveraging a recently developed assay for detection of caRNAs and their genomic association, we report that mitochondrial RNAs (mtRNAs) are attached to the nuclear genome and constitute a subset of caRNA, thus termed mt-caRNA. In four human cell types analyzed, mt-caRNAs preferentially attach to promoter regions. In human endothelial cells (ECs), the level of mt-caRNA-promoter attachment changes in response to environmental stress that mimics diabetes. Suppression of a non-coding mt-caRNA in ECs attenuates stress-induced nascent RNA transcription from the nuclear genome, including that of critical genes regulating cell adhesion, and abolishes stress-induced monocyte adhesion, a hallmark of dysfunctional ECs. Finally, we report increased nuclear localization of multiple mtRNAs in the ECs of human diabetic donors, suggesting many mtRNA translocate to the nucleus in a cell stress and disease-dependent manner. These data nominate mt-caRNAs as messenger molecules responsible for mitochondrial-nuclear communication and connect the immediate product of mitochondrial transcription with the transcriptional regulation of the nuclear genome.","journal":"eLife","year":2023,"id":339123,"datarank":0.6246447272068945,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"self_citation_contribution":0.4335557636844247,"citation_network_contribution":0.19108896352246968,"self_endowment_contribution":0.4335557636844247,"citer_contribution":0.19108896352246968,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":13,"citers_with_citation_signal":12,"citers_with_endowment":12,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9574,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE211971","GSE135357"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":808842,"name":"Zhijie Qi","orcid":"0000-0002-0022-8844","position":1,"is_corresponding":false},{"id":875619,"name":"Dongqiang Yuan","orcid":null,"position":2,"is_corresponding":false},{"id":791053,"name":"Naseeb Kaur Malhi","orcid":"0000-0002-8981-2974","position":3,"is_corresponding":false},{"id":344928,"name":"Xuejing Liu","orcid":"0000-0002-3931-0191","position":4,"is_corresponding":false},{"id":303736,"name":"Riccardo Calandrelli","orcid":"0000-0002-4541-6320","position":5,"is_corresponding":false},{"id":297549,"name":"Yingjun Luo","orcid":"0000-0003-2271-3725","position":6,"is_corresponding":false},{"id":874969,"name":"Alonso Tapia","orcid":"0000-0002-3123-9159","position":7,"is_corresponding":false},{"id":666342,"name":"Shengyan Jin","orcid":"0000-0002-8667-7586","position":8,"is_corresponding":false},{"id":1011761,"name":"Ji Shi","orcid":"0009-0006-0983-1419","position":9,"is_corresponding":false},{"id":322440,"name":"Martha Salas","orcid":"0009-0006-0159-5896","position":10,"is_corresponding":false},{"id":1012295,"name":"Runrui Dang","orcid":null,"position":11,"is_corresponding":false},{"id":455017,"name":"Brian Armstrong","orcid":"0009-0003-5026-2631","position":12,"is_corresponding":false},{"id":457033,"name":"Saul J. Priceman","orcid":"0000-0002-8136-2112","position":13,"is_corresponding":false},{"id":459926,"name":"Ping H. Wang","orcid":"0000-0002-7847-7752","position":14,"is_corresponding":false},{"id":430097,"name":"Jiayu Liao","orcid":"0000-0002-2624-5781","position":15,"is_corresponding":false},{"id":283024,"name":"Rama Natarajan","orcid":"0000-0003-4494-1788","position":16,"is_corresponding":false},{"id":90397,"name":"Sheng Zhong","orcid":"0000-0001-6419-7453","position":17,"is_corresponding":false},{"id":297552,"name":"Zhen Chen","orcid":"0000-0002-3291-1090","position":18,"is_corresponding":false},{"id":303739,"name":"Kiran Sriram","orcid":"0000-0002-7412-2380","position":0,"is_corresponding":true}],"reference_count":64,"raw_metadata":null,"created_at":"2026-07-19T01:10:45.048445Z","pmid":"38251974","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}