{"doi":"10.7554/elife.85056","title":"Fluorescein-based sensors to purify human α-cells for functional and transcriptomic analyses","abstract":"Pancreatic α-cells secrete glucagon, an insulin counter-regulatory peptide hormone critical for the maintenance of glucose homeostasis. Investigation of the function of human α-cells remains a challenge due to the lack of cost-effective purification methods to isolate high-quality α-cells from islets. Here, we use the reaction-based probe diacetylated Zinpyr1 (DA-ZP1) to introduce a novel and simple method for enriching live α-cells from dissociated human islet cells with ~95% purity. The α-cells, confirmed by sorting and immunostaining for glucagon, were cultured up to 10 days to form α-pseudoislets. The α-pseudoislets could be maintained in culture without significant loss of viability, and responded to glucose challenge by secreting appropriate levels of glucagon. RNA-sequencing analyses (RNA-seq) revealed that expression levels of key α-cell identity genes were sustained in culture while some of the genes such as DLK1 , GSN , SMIM24 were altered in α-pseudoislets in a time-dependent manner. In conclusion, we report a method to sort human primary α-cells with high purity that can be used for downstream analyses such as functional and transcriptional studies.","journal":"eLife","year":2023,"id":386740,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9573,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE199412","GSE84133"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":748783,"name":"Kimitaka Shibue","orcid":"0000-0002-4576-4739","position":1,"is_corresponding":false},{"id":294804,"name":"Dario F. De Jesus","orcid":"0000-0001-8826-9095","position":2,"is_corresponding":false},{"id":379200,"name":"Hyunki Kim","orcid":"0000-0003-2292-5584","position":3,"is_corresponding":false},{"id":748784,"name":"Jiang Hu","orcid":"0000-0003-4284-2575","position":4,"is_corresponding":false},{"id":750706,"name":"Debasish Manna","orcid":"0000-0003-4446-7318","position":5,"is_corresponding":false},{"id":349799,"name":"Bridget K. Wagner","orcid":"0000-0002-2629-361X","position":6,"is_corresponding":false},{"id":275621,"name":"Amit Choudhary","orcid":"0000-0002-8437-0150","position":7,"is_corresponding":false},{"id":269959,"name":"Rohit Kulkarni","orcid":"0000-0001-5029-6119","position":8,"is_corresponding":false},{"id":530588,"name":"Sevim Kahraman","orcid":"0000-0002-2880-6589","position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":null,"created_at":"2026-07-19T01:18:00.717763Z","pmid":"37732504","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}