{"doi":"10.7554/elife.84853","title":"Retinal microvascular and neuronal pathologies probed in vivo by adaptive optical two-photon fluorescence microscopy","abstract":"The retina, behind the transparent optics of the eye, is the only neural tissue whose physiology and pathology can be non-invasively probed by optical microscopy. The aberrations intrinsic to the mouse eye, however, prevent high-resolution investigation of retinal structure and function in vivo. Optimizing the design of a two-photon fluorescence microscope (2PFM) and sample preparation procedure, we found that adaptive optics (AO), by measuring and correcting ocular aberrations, is essential for resolving putative synaptic structures and achieving three-dimensional cellular resolution in the mouse retina in vivo. Applying AO-2PFM to longitudinal retinal imaging in transgenic models of retinal pathology, we characterized microvascular lesions with sub-capillary details in a proliferative vascular retinopathy model, and found Lidocaine to effectively suppress retinal ganglion cell hyperactivity in a retinal degeneration model. Tracking structural and functional changes at high-resolution longitudinally, AO-2PFM enables microscopic investigations of retinal pathology and pharmacology for disease diagnosis and treatment in vivo.","journal":"eLife","year":2023,"id":330355,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9571,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":395663,"name":"Yuhan Yang","orcid":"0009-0001-3845-1988","position":1,"is_corresponding":false},{"id":638403,"name":"Kevin Cao","orcid":"0000-0002-0226-7687","position":2,"is_corresponding":false},{"id":395664,"name":"Wei Chen","orcid":"0000-0001-5451-6430","position":3,"is_corresponding":false},{"id":998365,"name":"Santosh Kumar Paidi","orcid":"0000-0002-7034-586X","position":4,"is_corresponding":false},{"id":778807,"name":"Chun‐hong Xia","orcid":null,"position":5,"is_corresponding":false},{"id":288676,"name":"Richard Krämer","orcid":"0000-0002-8755-9389","position":6,"is_corresponding":false},{"id":778197,"name":"Xiaohua Gong","orcid":"0000-0003-2074-1802","position":7,"is_corresponding":false},{"id":260706,"name":"Na Ji","orcid":"0000-0002-5527-1663","position":8,"is_corresponding":false},{"id":260699,"name":"Qinrong Zhang","orcid":"0000-0001-8893-514X","position":0,"is_corresponding":true}],"reference_count":78,"raw_metadata":null,"created_at":"2026-07-19T01:09:10.120703Z","pmid":"37039777","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}