{"doi":"10.7554/elife.77595","title":"The ACF chromatin-remodeling complex is essential for Polycomb repression","abstract":"Establishing and maintaining appropriate gene repression is critical for the health and development of multicellular organisms. Histone H3 lysine 27 (H3K27) methylation is a chromatin modification associated with repressed facultative heterochromatin, but the mechanism of this repression remains unclear. We used a forward genetic approach to identify genes involved in transcriptional silencing of H3K27-methylated chromatin in the filamentous fungus Neurospora crassa . We found that the N. crassa homologs of ISWI (NCU03875) and ACF1 (NCU00164) are required for repression of a subset of H3K27-methylated genes and that they form an ACF chromatin-remodeling complex. This ACF complex interacts with chromatin throughout the genome, yet association with facultative heterochromatin is specifically promoted by the H3K27 methyltransferase, SET-7. H3K27-methylated genes that are upregulated when iswi or acf1 are deleted show a downstream shift of the +1 nucleosome, suggesting that proper nucleosome positioning is critical for repression of facultative heterochromatin. Our findings support a direct role of the ACF complex in Polycomb repression.","journal":"eLife","year":2022,"id":250425,"datarank":0.86690750211209,"base_score":3.295836866004329,"endowment":3.295836866004329,"self_citation_contribution":0.4943755299006494,"citation_network_contribution":0.3725319722114405,"self_endowment_contribution":0.4943755299006494,"citer_contribution":0.3725319722114405,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":23,"citers_with_citation_signal":16,"citers_with_endowment":16,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9585,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE168277"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":827934,"name":"Colleen C. Mumford","orcid":"0000-0003-0213-0901","position":1,"is_corresponding":false},{"id":337808,"name":"Kevin J. McNaught","orcid":"0000-0002-6887-3161","position":2,"is_corresponding":false},{"id":191312,"name":"Hideki Tanizawa","orcid":"0000-0002-2573-2473","position":3,"is_corresponding":false},{"id":337810,"name":"Eric U. Selker","orcid":"0000-0001-6465-0094","position":4,"is_corresponding":false},{"id":337809,"name":"Elizabeth T. Wiles","orcid":"0000-0001-7269-7466","position":0,"is_corresponding":true}],"reference_count":68,"raw_metadata":null,"created_at":"2026-07-19T00:24:28.242323Z","pmid":"35257662","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}