{"doi":"10.7554/elife.75433","title":"Conformational dynamics and allosteric modulation of the SARS-CoV-2 spike","abstract":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects cells through binding to angiotensin-converting enzyme 2 (ACE2). This interaction is mediated by the receptor-binding domain (RBD) of the viral spike (S) glycoprotein. Structural and dynamic data have shown that S can adopt multiple conformations, which controls the exposure of the ACE2-binding site in the RBD. Here, using single-molecule Förster resonance energy transfer (smFRET) imaging, we report the effects of ACE2 and antibody binding on the conformational dynamics of S from the Wuhan-1 strain and in the presence of the D614G mutation. We find that D614G modulates the energetics of the RBD position in a manner similar to ACE2 binding. We also find that antibodies that target diverse epitopes, including those distal to the RBD, stabilize the RBD in a position competent for ACE2 binding. Parallel solution-based binding experiments using fluorescence correlation spectroscopy (FCS) indicate antibody-mediated enhancement of ACE2 binding. These findings inform on novel strategies for therapeutic antibody cocktails.","journal":"eLife","year":2022,"id":234475,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":97,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9621,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":249881,"name":"Qi Li","orcid":"0000-0003-2112-8452","position":1,"is_corresponding":false},{"id":249880,"name":"Monir Ejemel","orcid":"0000-0001-9155-5914","position":2,"is_corresponding":false},{"id":550565,"name":"Leonid Yurkovetskiy","orcid":"0000-0001-5078-493X","position":3,"is_corresponding":false},{"id":284590,"name":"Jeremy Luban","orcid":"0000-0001-5650-4054","position":4,"is_corresponding":false},{"id":189209,"name":"Kuang Shen","orcid":null,"position":5,"is_corresponding":false},{"id":106266,"name":"Yang Wang","orcid":"0009-0001-2403-1448","position":6,"is_corresponding":false},{"id":284591,"name":"James B. Munro","orcid":"0000-0001-7634-4633","position":7,"is_corresponding":false},{"id":850068,"name":"Marco A Díaz-Salinas","orcid":"0000-0003-2983-0123","position":0,"is_corresponding":true}],"reference_count":68,"raw_metadata":null,"created_at":"2026-07-19T00:21:36.529874Z","pmid":"35323111","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}