{"doi":"10.7554/elife.73220","title":"Legionella pneumophila regulates host cell motility by targeting Phldb2 with a 14-3-3ζ-dependent protease effector","abstract":"The cytoskeleton network of eukaryotic cells is essential for diverse cellular processes, including vesicle trafficking, cell motility, and immunity, thus is a common target for bacterial virulence factors. A number of effectors from the bacterial pathogen Legionella pneumophila have been shown to modulate the function of host actin cytoskeleton to construct the Legionella -containing vacuole (LCV) permissive for its intracellular replication. In this study, we found that the Dot/Icm effector Lem8 (Lpg1290) is a protease whose activity is catalyzed by a Cys-His-Asp motif known to be associated with diverse biochemical activities. Intriguingly, we found that Lem8 interacts with the host regulatory protein 14-3-3ζ, which activates its protease activity. Furthermore, Lem8 undergoes self-cleavage in a process that requires 14-3-3ζ. We identified the Pleckstrin homology-like domain-containing protein Phldb2 involved in cytoskeleton organization as a target of Lem8 and demonstrated that Lem8 plays a role in the inhibition of host cell migration by attacking Phldb2.","journal":"eLife","year":2022,"id":248039,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":31,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9633,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":673937,"name":"Jingjing Luo","orcid":"0000-0002-4841-2624","position":1,"is_corresponding":false},{"id":886734,"name":"Hongou Wang","orcid":"0000-0003-2264-8628","position":2,"is_corresponding":false},{"id":308970,"name":"Dan Huang","orcid":"0000-0001-9079-0955","position":3,"is_corresponding":false},{"id":829556,"name":"Yunhao Tan","orcid":"0000-0002-5516-4980","position":4,"is_corresponding":false},{"id":729690,"name":"Yao Liu","orcid":"0000-0002-4330-2389","position":5,"is_corresponding":false},{"id":886735,"name":"Yingwu Wang","orcid":"0000-0002-6201-3574","position":6,"is_corresponding":false},{"id":110342,"name":"Kaiwen Yu","orcid":"0000-0002-6050-8477","position":7,"is_corresponding":false},{"id":586998,"name":"Yong Zhang","orcid":"0000-0001-9811-3019","position":8,"is_corresponding":false},{"id":829558,"name":"Xiaoyun Liu","orcid":"0000-0001-7083-5263","position":9,"is_corresponding":false},{"id":829559,"name":"Dan Li","orcid":"0000-0002-3036-2374","position":10,"is_corresponding":false},{"id":395235,"name":"Zhao‐Qing Luo","orcid":"0000-0001-8890-6621","position":11,"is_corresponding":false},{"id":619301,"name":"Lei Song","orcid":"0000-0002-4115-065X","position":0,"is_corresponding":true}],"reference_count":94,"raw_metadata":null,"created_at":"2026-07-19T00:24:09.893688Z","pmid":"35175192","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}