{"doi":"10.7554/elife.72216","title":"5′-Modifications improve potency and efficacy of DNA donors for precision genome editing","abstract":"Nuclease-directed genome editing is a powerful tool for investigating physiology and has great promise as a therapeutic approach to correct mutations that cause disease. In its most precise form, genome editing can use cellular homology-directed repair (HDR) pathways to insert information from an exogenously supplied DNA-repair template (donor) directly into a targeted genomic location. Unfortunately, particularly for long insertions, toxicity and delivery considerations associated with repair template DNA can limit HDR efficacy. Here, we explore chemical modifications to both double-stranded and single-stranded DNA-repair templates. We describe 5′-terminal modifications, including in its simplest form the incorporation of triethylene glycol (TEG) moieties, that consistently increase the frequency of precision editing in the germlines of three animal models ( Caenorhabditis elegans , zebrafish, mice) and in cultured human cells.","journal":"eLife","year":2021,"id":152963,"datarank":2.1427748039795333,"base_score":4.290459441148391,"endowment":4.290459441148391,"self_citation_contribution":0.6435689161722588,"citation_network_contribution":1.4992058878072745,"self_endowment_contribution":0.6435689161722588,"citer_contribution":1.4992058878072745,"corpus_percentile":null,"corpus_rank":null,"citation_count":72,"citer_count":62,"citers_with_citation_signal":51,"citers_with_endowment":51,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9501,"is_data_producer":true,"deposit_databanks":{"Dryad":["10.5061/dryad.f7m0cfxwr"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":633971,"name":"Zexiang Chen","orcid":"0000-0002-1584-2417","position":1,"is_corresponding":false},{"id":620177,"name":"Aamir Mir","orcid":"0000-0003-0144-3914","position":2,"is_corresponding":false},{"id":649393,"name":"Gregoriy A. Dokshin","orcid":"0000-0002-8780-7143","position":3,"is_corresponding":false},{"id":650493,"name":"Pranathi M. Krishnamurthy","orcid":null,"position":4,"is_corresponding":false},{"id":397529,"name":"Yeonsoo Yoon","orcid":"0000-0002-4302-9933","position":5,"is_corresponding":false},{"id":576760,"name":"Judith Gallant","orcid":null,"position":6,"is_corresponding":false},{"id":586850,"name":"Ping Xu","orcid":"0000-0003-4331-8839","position":7,"is_corresponding":false},{"id":43291,"name":"Xiao‐Ou Zhang","orcid":"0000-0002-2027-1313","position":8,"is_corresponding":false},{"id":650494,"name":"Ahmet R. Ozturk","orcid":null,"position":9,"is_corresponding":false},{"id":649394,"name":"Masahiro Shin","orcid":"0000-0002-0231-894X","position":10,"is_corresponding":false},{"id":453068,"name":"Feston Idrizi","orcid":"0000-0002-8035-3951","position":11,"is_corresponding":false},{"id":306037,"name":"Pengpeng Liu","orcid":"0000-0002-4891-8780","position":12,"is_corresponding":false},{"id":649395,"name":"Hassan Gneid","orcid":"0000-0001-9326-2023","position":13,"is_corresponding":false},{"id":649396,"name":"Alireza Edraki","orcid":"0000-0003-0849-4888","position":14,"is_corresponding":false},{"id":304178,"name":"Nathan D. Lawson","orcid":"0000-0001-7788-9619","position":15,"is_corresponding":false},{"id":397531,"name":"Jaime A. Rivera‐Pérez","orcid":"0000-0001-5776-6291","position":16,"is_corresponding":false},{"id":254500,"name":"Erik J. Sontheimer","orcid":"0000-0002-0881-0310","position":17,"is_corresponding":false},{"id":348431,"name":"Jonathan K. Watts","orcid":"0000-0001-5706-1734","position":18,"is_corresponding":false},{"id":55773,"name":"Craig C. Mello","orcid":"0000-0001-9176-6551","position":19,"is_corresponding":false},{"id":563089,"name":"Krishna S. Ghanta","orcid":"0000-0001-7502-3141","position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":null,"created_at":"2026-07-18T23:43:39.820288Z","pmid":"34665130","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}