{"doi":"10.7554/elife.70881","title":"A light-gated transcriptional recorder for detecting cell-cell contacts","abstract":"Technologies for detecting cell-cell contacts are powerful tools for studying a wide range of biological processes, from neuronal signaling to cancer-immune interactions within the tumor microenvironment. Here, we report TRACC (Transcriptional Readout Activated by Cell-cell Contacts), a GPCR-based transcriptional recorder of cellular contacts, which converts contact events into stable transgene expression. TRACC is derived from our previous protein-protein interaction recorders, SPARK (Kim et al., 2017) and SPARK2 (Kim et al., 2019), reported in this journal. TRACC incorporates light gating via the light-oxygen-voltage-sensing (LOV) domain, which provides user-defined temporal control of tool activation and reduces background. We show that TRACC detects cell-cell contacts with high specificity and sensitivity in mammalian cell culture and that it can be used to interrogate interactions between neurons and glioma, a form of brain cancer.","journal":"eLife","year":2022,"id":262936,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9525,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":919954,"name":"Shawn M Gillespie","orcid":null,"position":1,"is_corresponding":false},{"id":919955,"name":"Nicholas A Kalogriopoulos","orcid":null,"position":2,"is_corresponding":false},{"id":919956,"name":"Michael A Quezada","orcid":null,"position":3,"is_corresponding":false},{"id":919957,"name":"Martin Jacko","orcid":null,"position":4,"is_corresponding":false},{"id":17105,"name":"Michelle Monje","orcid":"0000-0002-3547-237X","position":5,"is_corresponding":false},{"id":242245,"name":"Alice Y. Ting","orcid":"0000-0002-8277-5226","position":6,"is_corresponding":false},{"id":552755,"name":"Kelvin F. Cho","orcid":"0000-0003-2917-9713","position":0,"is_corresponding":true}],"reference_count":31,"raw_metadata":null,"created_at":"2026-07-19T00:26:29.321923Z","pmid":"35311648","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}