{"doi":"10.7554/elife.70619","title":"Loss of N1-methylation of G37 in tRNA induces ribosome stalling and reprograms gene expression","abstract":"N 1 -methylation of G37 is required for a subset of tRNAs to maintain the translational reading-frame. While loss of m 1 G37 increases ribosomal +1 frameshifting, whether it incurs additional translational defects is unknown. Here, we address this question by applying ribosome profiling to gain a genome-wide view of the effects of m 1 G37 deficiency on protein synthesis. Using E coli as a model, we show that m 1 G37 deficiency induces ribosome stalling at codons that are normally translated by m 1 G37-containing tRNAs. Stalling occurs during decoding of affected codons at the ribosomal A site, indicating a distinct mechanism than that of +1 frameshifting, which occurs after the affected codons leave the A site. Enzyme- and cell-based assays show that m 1 G37 deficiency reduces tRNA aminoacylation and in some cases peptide-bond formation. We observe changes of gene expression in m 1 G37 deficiency similar to those in the stringent response that is typically induced by deficiency of amino acids. This work demonstrates a previously unrecognized function of m 1 G37 that emphasizes its role throughout the entire elongation cycle of protein synthesis, providing new insight into its essentiality for bacterial growth and survival.","journal":"eLife","year":2021,"id":159477,"datarank":0.9193299872044826,"base_score":3.7376696182833684,"endowment":3.7376696182833684,"self_citation_contribution":0.5606504427425053,"citation_network_contribution":0.35867954446197725,"self_endowment_contribution":0.5606504427425053,"citer_contribution":0.35867954446197725,"corpus_percentile":null,"corpus_rank":null,"citation_count":41,"citer_count":24,"citers_with_citation_signal":19,"citers_with_endowment":19,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9545,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE165592"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":671483,"name":"Jae-Yeon Hwang","orcid":null,"position":1,"is_corresponding":false},{"id":671484,"name":"Thomas Christian","orcid":null,"position":2,"is_corresponding":false},{"id":451352,"name":"Sunita Maharjan","orcid":"0000-0002-7099-1802","position":3,"is_corresponding":false},{"id":289819,"name":"Fuad Mohammad","orcid":"0000-0002-0589-5216","position":4,"is_corresponding":false},{"id":488621,"name":"Howard Gamper","orcid":"0000-0001-9007-8546","position":5,"is_corresponding":false},{"id":289820,"name":"Allen R. Buskirk","orcid":"0000-0003-2720-6896","position":6,"is_corresponding":false},{"id":17829,"name":"Ya-Ming Hou","orcid":"0000-0001-6546-2597","position":7,"is_corresponding":false},{"id":396902,"name":"Isao Masuda","orcid":"0000-0001-9385-4424","position":0,"is_corresponding":true}],"reference_count":81,"raw_metadata":null,"created_at":"2026-07-18T23:44:39.550981Z","pmid":"34382933","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}