{"doi":"10.7554/elife.67598","title":"An auxin-inducible, GAL4-compatible, gene expression system for Drosophila","abstract":"The ability to control transgene expression, both spatially and temporally, is essential for studying model organisms. In Drosophila , spatial control is primarily provided by the GAL4/UAS system, whilst temporal control relies on a temperature-sensitive GAL80 (which inhibits GAL4) and drug-inducible systems. However, these are not ideal. Shifting temperature can impact on many physiological and behavioural traits, and the current drug-inducible systems are either leaky, toxic, incompatible with existing GAL4-driver lines, or do not generate effective levels of expression. Here, we describe the auxin-inducible gene expression system (AGES). AGES relies on the auxin-dependent degradation of a ubiquitously expressed GAL80, and therefore, is compatible with existing GAL4-driver lines. Water-soluble auxin is added to fly food at a low, non-lethal, concentration, which induces expression comparable to uninhibited GAL4 expression. The system works in both larvae and adults, providing a stringent, non-lethal, cost-effective, and convenient method for temporally controlling GAL4 activity in Drosophila .","journal":"eLife","year":2022,"id":237193,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":62,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9502,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":409327,"name":"Amira Hassan","orcid":"0000-0003-1640-1602","position":1,"is_corresponding":false},{"id":502687,"name":"Gabriel Aughey","orcid":"0000-0001-5610-9345","position":2,"is_corresponding":false},{"id":859753,"name":"Khushbakht Butt","orcid":null,"position":3,"is_corresponding":false},{"id":409326,"name":"Alicia Estacio‐Gómez","orcid":"0000-0001-6571-0757","position":4,"is_corresponding":false},{"id":859754,"name":"Aneisha Duggal","orcid":null,"position":5,"is_corresponding":false},{"id":859755,"name":"Chee Ying Sia","orcid":null,"position":6,"is_corresponding":false},{"id":568955,"name":"Annika F. Barber","orcid":"0000-0002-5058-939X","position":7,"is_corresponding":false},{"id":409329,"name":"Tony D. Southall","orcid":"0000-0002-8645-4198","position":8,"is_corresponding":false},{"id":859028,"name":"Colin D. McClure","orcid":"0000-0001-6298-5296","position":0,"is_corresponding":true}],"reference_count":67,"raw_metadata":null,"created_at":"2026-07-19T00:22:08.925400Z","pmid":"35363137","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}