{"doi":"10.7554/elife.67321","title":"Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53","abstract":"During HIV-1 particle formation, the requisite plasma membrane curvature is thought to be solely driven by the retroviral Gag protein. Here, we reveal that the cellular I-BAR protein IRSp53 is required for the progression of HIV-1 membrane curvature to complete particle assembly. siRNA-mediated knockdown of IRSp53 gene expression induces a decrease in viral particle production and a viral bud arrest at half completion. Single-molecule localization microscopy at the cell plasma membrane shows a preferential localization of IRSp53 around HIV-1 Gag assembly sites. In addition, we observe the presence of IRSp53 in purified HIV-1 particles. Finally, HIV-1 Gag protein preferentially localizes to curved membranes induced by IRSp53 I-BAR domain on giant unilamellar vesicles. Overall, our data reveal a strong interplay between IRSp53 I-BAR and Gag at membranes during virus assembly. This highlights IRSp53 as a crucial host factor in HIV-1 membrane curvature and its requirement for full HIV-1 particle assembly.","journal":"eLife","year":2021,"id":159230,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":42,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9581,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":669599,"name":"Feng‐Ching Tsai","orcid":"0000-0002-6869-5254","position":1,"is_corresponding":false},{"id":669600,"name":"Rayane Dibsy","orcid":"0000-0003-3475-7389","position":2,"is_corresponding":false},{"id":670195,"name":"Aurore de Poret","orcid":null,"position":3,"is_corresponding":false},{"id":669601,"name":"John Manzi","orcid":"0000-0003-2574-4260","position":4,"is_corresponding":false},{"id":670196,"name":"Peggy Mérida","orcid":null,"position":5,"is_corresponding":false},{"id":669602,"name":"R. Müller","orcid":"0000-0003-4264-6590","position":6,"is_corresponding":false},{"id":460720,"name":"Pekka Lappalainen","orcid":"0000-0001-6227-0354","position":7,"is_corresponding":false},{"id":669603,"name":"Philippe Roingeard","orcid":"0000-0001-9131-3341","position":8,"is_corresponding":false},{"id":669604,"name":"Johnson Mak","orcid":"0000-0002-5229-5707","position":9,"is_corresponding":false},{"id":484741,"name":"Patricia Bassereau","orcid":"0000-0002-8544-6778","position":10,"is_corresponding":false},{"id":669605,"name":"Cyril Favard","orcid":"0000-0002-8304-2980","position":11,"is_corresponding":false},{"id":669606,"name":"Delphine Muriaux","orcid":"0000-0001-8517-9342","position":12,"is_corresponding":false},{"id":669598,"name":"Kaushik Inamdar","orcid":"0000-0001-5959-6409","position":0,"is_corresponding":true}],"reference_count":59,"raw_metadata":null,"created_at":"2026-07-18T23:44:35.165422Z","pmid":"34114563","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}