{"doi":"10.7554/elife.67301","title":"Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm","abstract":"Implant-associated Staphylococcus aureus infections are difficult to treat because of biofilm formation. Bacteria in a biofilm are often insensitive to antibiotics and host immunity. Monoclonal antibodies (mAbs) could provide an alternative approach to improve the diagnosis and potential treatment of biofilm-related infections. Here, we show that mAbs targeting common surface components of S. aureus can recognize clinically relevant biofilm types. The mAbs were also shown to bind a collection of clinical isolates derived from different biofilm-associated infections (endocarditis, prosthetic joint, catheter). We identify two groups of antibodies: one group that uniquely binds S. aureus in biofilm state and one that recognizes S. aureus in both biofilm and planktonic state. Furthermore, we show that a mAb recognizing wall teichoic acid (clone 4497) specifically localizes to a subcutaneously implanted pre-colonized catheter in mice. In conclusion, we demonstrate the capacity of several human mAbs to detect S. aureus biofilms in vitro and in vivo.","journal":"eLife","year":2021,"id":163713,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":41,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.96,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":684274,"name":"Bruce van Dijk","orcid":null,"position":1,"is_corresponding":false},{"id":684275,"name":"Kok van Kessel","orcid":null,"position":2,"is_corresponding":false},{"id":684276,"name":"Jeffrey S Kavanaugh","orcid":null,"position":3,"is_corresponding":false},{"id":684277,"name":"Carla de Haas","orcid":null,"position":4,"is_corresponding":false},{"id":684278,"name":"Piet C Aerts","orcid":null,"position":5,"is_corresponding":false},{"id":684279,"name":"Marco C Viveen","orcid":null,"position":6,"is_corresponding":false},{"id":683744,"name":"Edwin C Boel","orcid":"0000-0002-8506-0357","position":7,"is_corresponding":false},{"id":684280,"name":"Ad C Fluit","orcid":null,"position":8,"is_corresponding":false},{"id":230750,"name":"Jakub Kwieciński","orcid":"0000-0001-9472-2896","position":9,"is_corresponding":false},{"id":684281,"name":"Gerard C Krijger","orcid":null,"position":10,"is_corresponding":false},{"id":684282,"name":"Ruud M Ramakers","orcid":null,"position":11,"is_corresponding":false},{"id":684283,"name":"Freek J Beekman","orcid":null,"position":12,"is_corresponding":false},{"id":368608,"name":"Ekaterina Dadachova","orcid":"0000-0001-7300-6479","position":13,"is_corresponding":false},{"id":684284,"name":"Marnix GEH Lam","orcid":null,"position":14,"is_corresponding":false},{"id":684285,"name":"H Charles Vogely","orcid":null,"position":15,"is_corresponding":false},{"id":684286,"name":"Bart CH van der Wal","orcid":null,"position":16,"is_corresponding":false},{"id":684287,"name":"Jos AG van Strijp","orcid":null,"position":17,"is_corresponding":false},{"id":684288,"name":"Alexander R Horswill","orcid":null,"position":18,"is_corresponding":false},{"id":331496,"name":"Harrie Weinans","orcid":"0000-0002-2275-6170","position":19,"is_corresponding":false},{"id":683745,"name":"Suzan HM Rooijakkers","orcid":"0000-0003-4102-0377","position":20,"is_corresponding":false},{"id":683743,"name":"Lisanne de Vor","orcid":"0000-0002-2289-3378","position":0,"is_corresponding":true}],"reference_count":86,"raw_metadata":null,"created_at":"2026-07-18T23:45:22.477398Z","pmid":"34989676","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}