{"doi":"10.7554/elife.64904","title":"The Drosophila Nab2 RNA binding protein inhibits m6A methylation and male-specific splicing of Sex lethal transcript in female neuronal tissue","abstract":"The Drosophila polyadenosine RNA binding protein Nab2, which is orthologous to a human protein lost in a form of inherited intellectual disability, controls adult locomotion, axon projection, dendritic arborization, and memory through a largely undefined set of target RNAs. Here, we show a specific role for Nab2 in regulating splicing of ~150 exons/introns in the head transcriptome and focus on retention of a male-specific exon in the sex determination factor Sex-lethal ( Sxl ) that is enriched in female neurons. Previous studies have revealed that this splicing event is regulated in females by N6-methyladenosine (m 6 A) modification by the Mettl3 complex. At a molecular level, Nab2 associates with Sxl pre-mRNA in neurons and limits Sxl m 6 A methylation at specific sites. In parallel, reducing expression of the Mettl3, Mettl3 complex components, or the m 6 A reader Ythdc1 rescues mutant phenotypes in Nab2 flies. Overall, these data identify Nab2 as an inhibitor of m 6 A methylation and imply significant overlap between Nab2 and Mettl3 regulated RNAs in neuronal tissue.","journal":"eLife","year":2023,"id":341351,"datarank":0.5051123940419321,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.08922408570596488,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.08922408570596488,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":12,"citers_with_citation_signal":6,"citers_with_endowment":6,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9538,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE162531"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":496957,"name":"Carly L. Lancaster","orcid":"0000-0003-1195-9535","position":1,"is_corresponding":false},{"id":454992,"name":"J. Christopher Rounds","orcid":"0000-0001-5287-8454","position":2,"is_corresponding":false},{"id":494949,"name":"Brianna E Brown","orcid":null,"position":3,"is_corresponding":false},{"id":430727,"name":"Sara W. Leung","orcid":"0000-0001-5090-7995","position":4,"is_corresponding":false},{"id":565796,"name":"Ayan Banerjee","orcid":"0000-0001-8350-851X","position":5,"is_corresponding":false},{"id":494233,"name":"Derrick J. Morton","orcid":"0000-0001-6070-0904","position":6,"is_corresponding":false},{"id":566529,"name":"Rick S. Bienkowski","orcid":null,"position":7,"is_corresponding":false},{"id":471769,"name":"Milo B. Fasken","orcid":"0000-0003-4317-7909","position":8,"is_corresponding":false},{"id":295692,"name":"Isaac Kremsky","orcid":"0000-0003-1160-1373","position":9,"is_corresponding":false},{"id":958574,"name":"Matthew Tegowski","orcid":"0000-0003-1340-9254","position":10,"is_corresponding":false},{"id":318745,"name":"Kate D. Meyer","orcid":"0000-0001-7197-4054","position":11,"is_corresponding":false},{"id":430731,"name":"Anita H. Corbett","orcid":"0000-0002-0461-6895","position":12,"is_corresponding":false},{"id":443763,"name":"Kenneth H. Moberg","orcid":"0000-0002-9820-5543","position":13,"is_corresponding":false},{"id":494234,"name":"Binta Jalloh","orcid":"0000-0001-6475-3149","position":0,"is_corresponding":true}],"reference_count":88,"raw_metadata":null,"created_at":"2026-07-19T01:11:03.393978Z","pmid":"37458420","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}