{"doi":"10.7554/elife.64207","title":"Dynamic interactions between the RNA chaperone Hfq, small regulatory RNAs, and mRNAs in live bacterial cells","abstract":"RNA-binding proteins play myriad roles in regulating RNAs and RNA-mediated functions. In bacteria, the RNA chaperone Hfq is an important post-transcriptional gene regulator. Using live-cell super-resolution imaging, we can distinguish Hfq binding to different sizes of cellular RNAs. We demonstrate that under normal growth conditions, Hfq exhibits widespread mRNA-binding activity, with the distal face of Hfq contributing mostly to the mRNA binding in vivo. In addition, sRNAs can either co-occupy Hfq with the mRNA as a ternary complex, or displace the mRNA from Hfq in a binding face-dependent manner, suggesting mechanisms through which sRNAs rapidly access Hfq to induce sRNA-mediated gene regulation. Finally, our data suggest that binding of Hfq to certain mRNAs through its distal face can recruit RNase E to promote turnover of these mRNAs in a sRNA-independent manner, and such regulatory function of Hfq can be decoyed by sRNA competitors that bind strongly at the distal face.","journal":"eLife","year":2021,"id":156179,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":55,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9571,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":564218,"name":"Karine Prévost","orcid":null,"position":1,"is_corresponding":false},{"id":563785,"name":"Emily M. Heideman","orcid":"0000-0001-6563-8097","position":2,"is_corresponding":false},{"id":563786,"name":"Marie‐Claude Carrier","orcid":"0000-0002-3878-4141","position":3,"is_corresponding":false},{"id":530828,"name":"Muhammad S. Azam","orcid":"0000-0003-2312-6573","position":4,"is_corresponding":false},{"id":563787,"name":"Matthew Reyer","orcid":"0000-0002-9195-5720","position":5,"is_corresponding":false},{"id":660191,"name":"Wei Liu","orcid":"0000-0002-1207-204X","position":6,"is_corresponding":false},{"id":332325,"name":"Éric Massé","orcid":"0000-0002-5253-1415","position":7,"is_corresponding":false},{"id":535457,"name":"Jingyi Fei","orcid":"0000-0002-9775-3820","position":8,"is_corresponding":false},{"id":563784,"name":"Seong-Jin Park","orcid":"0000-0002-4298-1770","position":0,"is_corresponding":true}],"reference_count":86,"raw_metadata":null,"created_at":"2026-07-18T23:44:08.368845Z","pmid":"33616037","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}