{"doi":"10.7554/elife.63379","title":"Transsynaptic mapping of Drosophila mushroom body output neurons","abstract":"<jats:p>\n                    The mushroom body (MB) is a well-characterized associative memory structure within the\n                    <jats:italic>Drosophila</jats:italic>\n                    brain. Analyzing MB connectivity using multiple approaches is critical for understanding the functional implications of this structure. Using the genetic anterograde transsynaptic tracing tool,\n                    <jats:italic>trans-</jats:italic>\n                    Tango, we identified divergent projections across the brain and convergent downstream targets of the MB output neurons (MBONs). Our analysis revealed at least three separate targets that receive convergent input from MBONs: other MBONs, the fan-shaped body (FSB), and the lateral accessory lobe (LAL). We describe, both anatomically and functionally, a multilayer circuit in which inhibitory and excitatory MBONs converge on the same genetic subset of FSB and LAL neurons. This circuit architecture enables the brain to update and integrate information with previous experience before executing appropriate behavioral responses. Our use of\n                    <jats:italic>trans</jats:italic>\n                    -Tango provides a genetically accessible anatomical framework for investigating the functional relevance of components within these complex and interconnected circuits.\n                  </jats:p>","journal":"eLife","year":2021,"id":673474,"datarank":0.6238324625039509,"base_score":4.1588830833596715,"endowment":4.1588830833596715,"self_citation_contribution":0.6238324625039509,"citation_network_contribution":0.0,"self_endowment_contribution":0.6238324625039509,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":63,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":334732,"name":"Mustafa Talay","orcid":"0000-0002-9046-1366","position":1,"is_corresponding":false},{"id":1759566,"name":"John D Fisher","orcid":null,"position":2,"is_corresponding":false},{"id":568272,"name":"Raphael Cohn","orcid":"0000-0002-6899-1475","position":3,"is_corresponding":false},{"id":568273,"name":"Altar Sorkaç","orcid":"0000-0002-0739-6314","position":4,"is_corresponding":false},{"id":228494,"name":"Yoshinori Aso","orcid":"0000-0002-2939-1688","position":5,"is_corresponding":false},{"id":15471,"name":"Gilad Barnea","orcid":"0000-0001-6842-3454","position":6,"is_corresponding":false},{"id":568274,"name":"Karla R. Kaun","orcid":"0000-0002-8756-9528","position":7,"is_corresponding":false},{"id":568271,"name":"Kristin M. Scaplen","orcid":"0000-0001-7493-1420","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Transsynaptic mapping of Drosophila mushroom body output neurons","abstract":"<jats:p>\n                    The mushroom body (MB) is a well-characterized associative memory structure within the\n                    <jats:italic>Drosophila</jats:italic>\n                    brain. Analyzing MB connectivity using multiple approaches is critical for understanding the functional implications of this structure. Using the genetic anterograde transsynaptic tracing tool,\n                    <jats:italic>trans-</jats:italic>\n                    Tango, we identified divergent projections across the brain and convergent downstream targets of the MB output neurons (MBONs). Our analysis revealed at least three separate targets that receive convergent input from MBONs: other MBONs, the fan-shaped body (FSB), and the lateral accessory lobe (LAL). We describe, both anatomically and functionally, a multilayer circuit in which inhibitory and excitatory MBONs converge on the same genetic subset of FSB and LAL neurons. This circuit architecture enables the brain to update and integrate information with previous experience before executing appropriate behavioral responses. Our use of\n                    <jats:italic>trans</jats:italic>\n                    -Tango provides a genetically accessible anatomical framework for investigating the functional relevance of components within these complex and interconnected circuits.\n                  </jats:p>","is_dataset_classified":null,"base_score":4.1588830833596715,"endowment":4.1588830833596715,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"33570489","pmcid":"PMC7877909","openalex_id":"https://openalex.org/W3128928323","authors":[],"funders":[{"funder_name":"National Institute on Alcohol Abuse and Alcoholism","grant_id":"R01AA024434","title":null},{"funder_name":"National Institute of General Medical Sciences","grant_id":"P20GM103645 (8278)","title":null},{"funder_name":"National Institute on Deafness and Other Communication Disorders","grant_id":"R01DC017146","title":null},{"funder_name":"National Institute of Mental Health","grant_id":"R01MH105368","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"P20 GM103645","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM098151","title":null},{"funder_name":"National Institutes of Health","grant_id":"5P20GM103645-02","title":"Development of vision and attention in typical and ASD individuals"},{"funder_name":"National Institutes of Health","grant_id":"5R01DC017146-03","title":"The neural circuits underlying gustatory perception in flies"},{"funder_name":"National Institutes of Health","grant_id":"1R01GM098151-01","title":"Fluorender: An Imaging Tool for Visualization and Analysis of Confocal Data as Ap"},{"funder_name":"National Institutes of Health","grant_id":"1R01MH105368-01","title":"Functional mapping of mammalian neural circuits"},{"funder_name":"National Institutes of Health","grant_id":"5R01AA024434-03","title":"Notch-dependent microcircuit regulation of alcohol reward memory"},{"funder_name":"Richard and Susan Smith Family Foundation","grant_id":"","title":null}],"total_grants":12,"fwci":4.5979,"citation_percentile":0.96212778,"influential_citations":0,"citation_trend":[{"year":2020,"count":2},{"year":2021,"count":10},{"year":2022,"count":13},{"year":2023,"count":9},{"year":2024,"count":16},{"year":2025,"count":11},{"year":2026,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.7554/elife.63379","host_type":"journal"},{"url":"https://doi.org/10.7554/elife.63379","host_type":"publisher"},{"url":"https://cdn.elifesciences.org/articles/63379/elife-63379-v3.pdf","host_type":"publisher"},{"url":"https://cdn.elifesciences.org/articles/63379/elife-63379-v3.xml","host_type":"publisher"},{"url":"https://elifesciences.org/articles/63379","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/33570489","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7877909","host_type":"repository"},{"url":"https://doaj.org/article/2d2c90be130240309c7463a2364c6d2a","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC7877909","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC7877909?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.1101/2020.09.22.309021","host_type":""},{"url":"https://www.biorxiv.org/content/biorxiv/early/2020/09/23/2020.09.22.309021.full.pdf","host_type":""},{"url":"http://dx.doi.org/10.7554/eLife.63379","host_type":""},{"url":"https://dx.doi.org/10.1101/2020.09.22.309021","host_type":""},{"url":"https://dx.doi.org/10.7554/elife.63379","host_type":""}],"fields_of_study":["Neurobiology and Insect Physiology Research","Physiological and biochemical adaptations","Zebrafish Biomedical Research Applications","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Animals","Drosophila melanogaster","Female","Male","Neurons","Mushroom Bodies"],"keywords":["Mushroom bodies","Mushroom","Drosophila (subgenus)","Biology","Neuroscience","Cell biology","Drosophila melanogaster","Genetics","Gene","Botany","Drosophila","Memory","Connectivity","Mushroom Body","D. Melanogaster","Trans-tango","Mushroom Body Output Neurons","Male","Neurons","QH301-705.5","Science","Q","R","Medicine","Animals","Female","Biology (General)"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T13:30:21.211211Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}