{"doi":"10.7554/elife.63274","title":"Efficient chromatin accessibility mapping in situ by nucleosome-tethered tagmentation","abstract":"Chromatin accessibility mapping is a powerful approach to identify potential regulatory elements. A popular example is ATAC-seq, whereby Tn5 transposase inserts sequencing adapters into accessible DNA ('tagmentation'). CUT&Tag is a tagmentation-based epigenomic profiling method in which antibody tethering of Tn5 to a chromatin epitope of interest profiles specific chromatin features in small samples and single cells. Here, we show that by simply modifying the tagmentation conditions for histone H3K4me2 or H3K4me3 CUT&Tag, antibody-tethered tagmentation of accessible DNA sites is redirected to produce chromatin accessibility maps that are indistinguishable from the best ATAC-seq maps. Thus, chromatin accessibility maps can be produced in parallel with CUT&Tag maps of other epitopes with all steps from nuclei to amplified sequencing-ready libraries performed in single PCR tubes in the laboratory or on a home workbench. As H3K4 methylation is produced by transcription at promoters and enhancers, our method identifies transcription-coupled accessible regulatory sites.","journal":"eLife","year":2020,"id":51160,"datarank":3.616938340920565,"base_score":5.117993812416755,"endowment":5.117993812416755,"self_citation_contribution":0.7676990718625134,"citation_network_contribution":2.8492392690580512,"self_endowment_contribution":0.7676990718625134,"citer_contribution":2.8492392690580512,"corpus_percentile":null,"corpus_rank":null,"citation_count":166,"citer_count":100,"citers_with_citation_signal":100,"citers_with_endowment":100,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9503,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE158327","GSE124557"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":256946,"name":"Jorja G. Henikoff","orcid":null,"position":1,"is_corresponding":false},{"id":256947,"name":"Hatice S Kaya-Okur","orcid":null,"position":2,"is_corresponding":false},{"id":45074,"name":"Kami Ahmad","orcid":"0000-0001-8572-6182","position":3,"is_corresponding":false},{"id":45084,"name":"Steven Henikoff","orcid":"0000-0002-7621-8685","position":0,"is_corresponding":true}],"reference_count":37,"raw_metadata":null,"created_at":"2026-07-18T20:40:28.278215Z","pmid":"33191916","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}