{"doi":"10.7554/elife.63102","title":"Stable transplantation of human mitochondrial DNA by high-throughput, pressurized isolated mitochondrial delivery","abstract":"Generating mammalian cells with specific mitochondrial DNA (mtDNA)–nuclear DNA (nDNA) combinations is desirable but difficult to achieve and would be enabling for studies of mitochondrial-nuclear communication and coordination in controlling cell fates and functions. We developed ‘MitoPunch’, a pressure-driven mitochondrial transfer device, to deliver isolated mitochondria into numerous target mammalian cells simultaneousl y . MitoPunch and MitoCeption, a previously described force-based mitochondrial transfer approach, both yield stable isolated mitochondrial recipient (SIMR) cells that permanently retain exogenous mtDNA, whereas coincubation of mitochondria with cells does not yield SIMR cells. Although a typical MitoPunch or MitoCeption delivery results in dozens of immortalized SIMR clones with restored oxidative phosphorylation, only MitoPunch can produce replication-limited, non-immortal human SIMR clones. The MitoPunch device is versatile, inexpensive to assemble, and easy to use for engineering mtDNA–nDNA combinations to enable fundamental studies and potential translational applications.","journal":"eLife","year":2021,"id":156153,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":55,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9564,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":329571,"name":"Alexander N. Patananan","orcid":"0000-0001-9458-9968","position":1,"is_corresponding":false},{"id":511607,"name":"Tianxing Man","orcid":"0000-0001-5079-3844","position":2,"is_corresponding":false},{"id":330450,"name":"Ting‐Hsiang Wu","orcid":null,"position":3,"is_corresponding":false},{"id":271155,"name":"Amy Yu","orcid":"0000-0003-2936-5225","position":4,"is_corresponding":false},{"id":568214,"name":"Garret W Guyot","orcid":null,"position":5,"is_corresponding":false},{"id":329581,"name":"Shahrooz Rabizadeh","orcid":"0000-0002-8609-7645","position":6,"is_corresponding":false},{"id":329582,"name":"Kayvan Niazi","orcid":"0000-0002-7098-1751","position":7,"is_corresponding":false},{"id":244627,"name":"Pei‐Yu Chiou","orcid":"0000-0003-4677-2447","position":8,"is_corresponding":false},{"id":244626,"name":"Michael A. Teitell","orcid":"0000-0002-4495-8750","position":9,"is_corresponding":false},{"id":329572,"name":"Alexander J. Sercel","orcid":"0000-0002-0749-2162","position":0,"is_corresponding":true}],"reference_count":33,"raw_metadata":null,"created_at":"2026-07-18T23:44:08.368845Z","pmid":"33438576","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}