{"doi":"10.7554/elife.62781","title":"Molecular tracking devices quantify antigen distribution and archiving in the murine lymph node","abstract":"The detection of foreign antigens in vivo has relied on fluorescent conjugation or indirect read-outs such as antigen presentation. In our studies, we found that these widely used techniques had several technical limitations that have precluded a complete picture of antigen trafficking or retention across lymph node cell types. To address these limitations, we developed a 'molecular tracking device' to follow the distribution, acquisition, and retention of antigen in the lymph node. Utilizing an antigen conjugated to a nuclease-resistant DNA tag, acting as a combined antigen-adjuvant conjugate, and single-cell mRNA sequencing, we quantified antigen abundance in the lymph node. Variable antigen levels enabled the identification of caveolar endocytosis as a mechanism of antigen acquisition or retention in lymphatic endothelial cells. Thus, these molecular tracking devices enable new approaches to study dynamic tissue dissemination of antigen-adjuvant conjugates and identify new mechanisms of antigen acquisition and retention at cellular resolution in vivo.","journal":"eLife","year":2021,"id":168536,"datarank":0.772544327902249,"base_score":3.4339872044851463,"endowment":3.4339872044851463,"self_citation_contribution":0.515098080672772,"citation_network_contribution":0.2574462472294771,"self_endowment_contribution":0.515098080672772,"citer_contribution":0.2574462472294771,"corpus_percentile":null,"corpus_rank":null,"citation_count":30,"citer_count":19,"citers_with_citation_signal":16,"citers_with_endowment":16,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9459,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE150719","GSE137710","GSE15907","GSE112903","GSE145121","GSE109129"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":225905,"name":"Ryan M. Sheridan","orcid":"0000-0003-4012-3147","position":1,"is_corresponding":false},{"id":327992,"name":"Erin D. Lucas","orcid":"0000-0001-7705-2069","position":2,"is_corresponding":false},{"id":697961,"name":"Thu A. Doan","orcid":"0000-0002-4838-0343","position":3,"is_corresponding":false},{"id":499382,"name":"Brian C. Ware","orcid":"0000-0001-7806-3456","position":4,"is_corresponding":false},{"id":327993,"name":"Johnathon Schafer","orcid":"0000-0001-8172-3953","position":5,"is_corresponding":false},{"id":225902,"name":"Rui Fu","orcid":"0000-0001-8183-4549","position":6,"is_corresponding":false},{"id":313598,"name":"Matthew A. Burchill","orcid":"0000-0003-3118-2962","position":7,"is_corresponding":false},{"id":87044,"name":"Jay R. Hesselberth","orcid":"0000-0002-6299-179X","position":8,"is_corresponding":false},{"id":251276,"name":"Beth A. Jirón Tamburini","orcid":"0000-0003-1991-231X","position":9,"is_corresponding":false},{"id":583536,"name":"Shannon M. Walsh","orcid":"0000-0001-9845-629X","position":0,"is_corresponding":true}],"reference_count":97,"raw_metadata":null,"created_at":"2026-07-18T23:46:06.853307Z","pmid":"33843587","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}