{"doi":"10.7554/elife.62312","title":"A Non-stop identity complex (NIC) supervises enterocyte identity and protects from premature aging","abstract":"midgut differentiated enterocytes (ECs) lose their identity, impairing tissue homeostasis. To discover identity regulators, we performed an RNAi screen targeting ubiquitin-related genes in ECs. Seventeen genes were identified, including the deubiquitinase Non-stop (CG4166). Lineage tracing established that acute loss of Non-stop in young ECs phenocopies aged ECs at cellular and tissue levels. Proteomic analysis unveiled that Non-stop maintains identity as part of a Non-stop identity complex (NIC) containing E(y)2, Sgf11, Cp190, (Mod) mdg4, and Nup98. Non-stop ensured chromatin accessibility, maintaining the EC-gene signature, and protected NIC subunit stability. Upon aging, the levels of Non-stop and NIC subunits declined, distorting the unique organization of the EC nucleus. Maintaining youthful levels of Non-stop in wildtype aged ECs safeguards NIC subunits, nuclear organization, and suppressed aging phenotypes. Thus, Non-stop and NIC, supervise EC identity and protects from premature aging.","journal":"eLife","year":2021,"id":183242,"datarank":0.5903365550025775,"base_score":2.639057329615259,"endowment":2.639057329615259,"self_citation_contribution":0.3958585994422889,"citation_network_contribution":0.1944779555602886,"self_endowment_contribution":0.3958585994422889,"citer_contribution":0.1944779555602886,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":9,"citers_with_citation_signal":8,"citers_with_endowment":8,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9596,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE87896","GSE112640"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":576582,"name":"Lena Israitel","orcid":null,"position":1,"is_corresponding":false},{"id":575857,"name":"Eliya Bitman-Lotan","orcid":"0000-0001-9174-6382","position":2,"is_corresponding":false},{"id":14218,"name":"Winghing Wong","orcid":"0000-0003-2494-1721","position":3,"is_corresponding":false},{"id":576583,"name":"Gal Raz","orcid":null,"position":4,"is_corresponding":false},{"id":736868,"name":"Dayanne V. Cornelio-Parra","orcid":null,"position":5,"is_corresponding":false},{"id":576584,"name":"Salwa Danial","orcid":null,"position":6,"is_corresponding":false},{"id":576585,"name":"Na’ama Flint Brodsly","orcid":null,"position":7,"is_corresponding":false},{"id":575858,"name":"Elena Belova","orcid":"0000-0003-2975-3906","position":8,"is_corresponding":false},{"id":329743,"name":"Oksana Maksimenko","orcid":"0000-0003-3502-0303","position":9,"is_corresponding":false},{"id":329748,"name":"Pavel Georgiev","orcid":"0000-0002-6509-3194","position":10,"is_corresponding":false},{"id":108814,"name":"Todd E. Druley","orcid":"0000-0002-3245-7561","position":11,"is_corresponding":false},{"id":575859,"name":"Ryan D. Mohan","orcid":"0000-0002-7624-4605","position":12,"is_corresponding":false},{"id":575860,"name":"Amir Orian","orcid":"0000-0002-8521-1661","position":13,"is_corresponding":false},{"id":267509,"name":"Neta Erez","orcid":"0000-0001-6506-9074","position":0,"is_corresponding":true}],"reference_count":82,"raw_metadata":null,"created_at":"2026-07-18T23:48:17.816498Z","pmid":"33629655","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}