{"doi":"10.7554/elife.60552","title":"Cryo-EM analysis of PIP2 regulation in mammalian GIRK channels","abstract":"G-protein-gated inward rectifier potassium (GIRK) channels are regulated by G proteins and PIP 2 . Here, using cryo-EM single particle analysis we describe the equilibrium ensemble of structures of neuronal GIRK2 as a function of the C8-PIP 2 concentration. We find that PIP 2 shifts the equilibrium between two distinguishable structures of neuronal GIRK (GIRK2), extended and docked, towards the docked form. In the docked form the cytoplasmic domain, to which G βγ binds, becomes accessible to the cytoplasmic membrane surface where G βγ resides. Furthermore, PIP 2 binding reshapes the G βγ binding surface on the cytoplasmic domain, preparing it to receive G βγ . We find that cardiac GIRK (GIRK1/4) can also exist in both extended and docked conformations. These findings lead us to conclude that PIP 2 influences GIRK channels in a structurally similar manner to Kir2.2 channels. In Kir2.2 channels, the PIP 2 -induced conformational changes open the pore. In GIRK channels, they prepare the channel for activation by G βγ .","journal":"eLife","year":2020,"id":56481,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":85,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9468,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":293947,"name":"Xiao Tao","orcid":"0000-0002-9381-7903","position":1,"is_corresponding":false},{"id":293948,"name":"Kouki K Touhara","orcid":"0000-0003-3167-9784","position":2,"is_corresponding":false},{"id":51699,"name":"Roderick MacKinnon","orcid":"0000-0001-7605-4679","position":3,"is_corresponding":false},{"id":293946,"name":"Yiming Niu","orcid":"0000-0002-5683-1781","position":0,"is_corresponding":true}],"reference_count":44,"raw_metadata":null,"created_at":"2026-07-18T21:06:09.605575Z","pmid":"32844743","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}