{"doi":"10.7554/elife.60480","title":"Chemical genetics and proteome-wide site mapping reveal cysteine MARylation by PARP-7 on immune-relevant protein targets","abstract":"Poly(ADP-ribose) polymerase 7 (PARP-7) has emerged as a critically important member of a large enzyme family that catalyzes ADP-ribosylation in mammalian cells. PARP-7 is a critical regulator of the innate immune response. What remains unclear is the mechanism by which PARP-7 regulates this process, namely because the protein targets of PARP-7 mono-ADP-ribosylation (MARylation) are largely unknown. Here, we combine chemical genetics, proximity labeling, and proteome-wide amino acid ADP-ribosylation site profiling for identifying the direct targets and sites of PARP-7-mediated MARylation in a cellular context. We found that the inactive PARP family member, PARP-13-a critical regulator of the antiviral innate immune response-is a major target of PARP-7. PARP-13 is preferentially MARylated on cysteine residues in its RNA binding zinc finger domain. Proteome-wide ADP-ribosylation analysis reveals cysteine as a major MARylation acceptor of PARP-7. This study provides insight into PARP-7 targeting and MARylation site preference.","journal":"eLife","year":2021,"id":151619,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":81,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9569,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":269801,"name":"Sara C. Buch-Larsen","orcid":"0000-0001-6250-5467","position":1,"is_corresponding":false},{"id":103651,"name":"Ilsa T. Kirby","orcid":"0000-0001-6120-7011","position":2,"is_corresponding":false},{"id":644452,"name":"Ivan Rodriguez Siordia","orcid":"0000-0001-7844-1349","position":3,"is_corresponding":false},{"id":645416,"name":"David Hutin","orcid":null,"position":4,"is_corresponding":false},{"id":644453,"name":"Marit Rasmussen","orcid":"0000-0003-0763-6725","position":5,"is_corresponding":false},{"id":434146,"name":"Denis M. Grant","orcid":"0000-0003-2834-0923","position":6,"is_corresponding":false},{"id":141412,"name":"Larry L. David","orcid":null,"position":7,"is_corresponding":false},{"id":618542,"name":"Jason Matthews","orcid":"0000-0002-9815-8636","position":8,"is_corresponding":false},{"id":50220,"name":"Michael L. Nielsen","orcid":"0000-0002-0067-9039","position":9,"is_corresponding":false},{"id":618528,"name":"Michael S. Cohen","orcid":"0000-0002-7636-4156","position":10,"is_corresponding":false},{"id":644451,"name":"Kelsie M. Rodriguez","orcid":"0000-0002-0821-6717","position":0,"is_corresponding":true}],"reference_count":64,"raw_metadata":null,"created_at":"2026-07-18T23:43:16.823357Z","pmid":"33475084","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}