{"doi":"10.7554/elife.59119","title":"Bardet–Biedl syndrome 3 protein promotes ciliary exit of the signaling protein phospholipase D via the BBSome","abstract":"Certain ciliary signaling proteins couple with the BBSome, a conserved complex of Bardet–Biedl syndrome (BBS) proteins, to load onto retrograde intraflagellar transport (IFT) trains for their removal out of cilia in Chlamydomonas reinhardtii . Here, we show that loss of the Arf-like 6 (ARL6) GTPase BBS3 causes the signaling protein phospholipase D (PLD) to accumulate in cilia. Upon targeting to the basal body, BBSomes enter and cycle through cilia via IFT, while BBS3 in a GTP-bound state separates from BBSomes, associates with the membrane, and translocates from the basal body to cilia by diffusion. Upon arriving at the ciliary tip, GTP-bound BBS3 binds and recruits BBSomes to the ciliary membrane for interacting with PLD, thus making the PLD-laden BBSomes available to load onto retrograde IFT trains for ciliary exit. Therefore, BBS3 promotes PLD exit from cilia via the BBSome, providing a regulatory mechanism for ciliary signaling protein removal out of cilia.","journal":"eLife","year":2021,"id":164104,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":39,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9501,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":309341,"name":"Bin Xue","orcid":"0000-0002-7256-1179","position":1,"is_corresponding":false},{"id":684874,"name":"Wei-Yue Sun","orcid":"0000-0002-1953-7698","position":2,"is_corresponding":false},{"id":309344,"name":"Jenna L. Wingfield","orcid":"0000-0002-9522-4183","position":3,"is_corresponding":false},{"id":309346,"name":"Jun Sun","orcid":"0000-0001-7369-7871","position":4,"is_corresponding":false},{"id":309345,"name":"Mingfu Wu","orcid":"0000-0003-0155-2328","position":5,"is_corresponding":false},{"id":309347,"name":"Karl F. Lechtreck","orcid":"0000-0002-6219-6470","position":6,"is_corresponding":false},{"id":684875,"name":"Zhenlong Wu","orcid":"0000-0001-9700-8874","position":7,"is_corresponding":false},{"id":309348,"name":"Zhen‐Chuan Fan","orcid":"0000-0003-1712-3413","position":8,"is_corresponding":false},{"id":309342,"name":"Yan‐Xia Liu","orcid":"0000-0003-4127-7148","position":0,"is_corresponding":true}],"reference_count":61,"raw_metadata":null,"created_at":"2026-07-18T23:45:27.031465Z","pmid":"33587040","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}