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We then transposed the cysteine mutation to the native receptor, to demonstrate with high pharmacological specificity that metabotropic glutamate receptor signaling triggers opening of GluD2. Our results assess the functional relevance of GluD2 ion channel and introduce an optogenetic tool that will provide a novel and powerful means for probing GluD2 ionotropic contribution to neuronal physiology.</jats:p>","journal":"eLife","year":2020,"id":678127,"datarank":0.5050943744979712,"base_score":3.367295829986474,"endowment":3.367295829986474,"self_citation_contribution":0.5050943744979712,"citation_network_contribution":0.0,"self_endowment_contribution":0.5050943744979712,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":28,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1381950,"name":"Sarah Mondoloni","orcid":"0000-0002-6134-3715","position":1,"is_corresponding":false},{"id":1771818,"name":"Jérome Tange","orcid":null,"position":2,"is_corresponding":false},{"id":1771819,"name":"Bertrand Lambolez","orcid":"0000-0002-0653-480X","position":3,"is_corresponding":false},{"id":301973,"name":"Philippe Fauré","orcid":"0000-0003-3573-4971","position":4,"is_corresponding":false},{"id":1725078,"name":"Antoine Taly","orcid":"0000-0001-5109-0091","position":5,"is_corresponding":false},{"id":1771820,"name":"Ludovic Tricoire","orcid":"0000-0003-3345-1468","position":6,"is_corresponding":false},{"id":1381954,"name":"Alexandre Mourot","orcid":"0000-0002-8839-7481","position":7,"is_corresponding":false},{"id":1771817,"name":"Damien Lemoine","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Probing the ionotropic activity of glutamate GluD2 receptor in HEK cells with genetically-engineered photopharmacology","abstract":"<jats:p>Glutamate delta (GluD) receptors belong to the ionotropic glutamate receptor family, yet they don’t bind glutamate and are considered orphan. 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