{"doi":"10.7554/elife.58882","title":"Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness","abstract":"Imaging neuronal activity with high and homogeneous spatial resolution across the field-of-view (FOV) and limited invasiveness in deep brain regions is fundamental for the progress of neuroscience, yet is a major technical challenge. We achieved this goal by correcting optical aberrations in gradient index lens-based ultrathin (≤500 µm) microendoscopes using aspheric microlenses generated through 3D-microprinting. Corrected microendoscopes had extended FOV ( eFOV ) with homogeneous spatial resolution for two-photon fluorescence imaging and required no modification of the optical set-up. Synthetic calcium imaging data showed that, compared to uncorrected endoscopes, eFOV -microendoscopes led to improved signal-to-noise ratio and more precise evaluation of correlated neuronal activity. We experimentally validated these predictions in awake head-fixed mice. Moreover, using eFOV- microendoscopes we demonstrated cell-specific encoding of behavioral state-dependent information in distributed functional subnetworks in a primary somatosensory thalamic nucleus. eFOV- microendoscopes are, therefore, small-cross-section ready-to-use tools for deep two-photon functional imaging with unprecedentedly high and homogeneous spatial resolution.","journal":"eLife","year":2020,"id":62149,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":53,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9556,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":328732,"name":"Andrea Sattin","orcid":"0000-0002-1345-2204","position":1,"is_corresponding":false},{"id":328733,"name":"Monica Moroni","orcid":"0000-0003-1852-7217","position":2,"is_corresponding":false},{"id":328734,"name":"Serena Bovetti","orcid":"0000-0002-0471-4081","position":3,"is_corresponding":false},{"id":328735,"name":"Claudio Moretti","orcid":"0000-0003-3314-8936","position":4,"is_corresponding":false},{"id":330202,"name":"Francesca Succol","orcid":null,"position":5,"is_corresponding":false},{"id":328736,"name":"Angelo Forli","orcid":"0000-0002-0857-029X","position":6,"is_corresponding":false},{"id":328737,"name":"Dania Vecchia","orcid":"0000-0002-6091-538X","position":7,"is_corresponding":false},{"id":330203,"name":"Vijayakumar P. Rajamanickam","orcid":null,"position":8,"is_corresponding":false},{"id":328738,"name":"Andrea Bertoncini","orcid":"0000-0001-8204-811X","position":9,"is_corresponding":false},{"id":249867,"name":"Stefano Panzeri","orcid":"0000-0003-1700-8909","position":10,"is_corresponding":false},{"id":328739,"name":"Carlo Liberale","orcid":"0000-0002-5653-199X","position":11,"is_corresponding":false},{"id":328740,"name":"Tommaso Fellin","orcid":"0000-0003-2718-7533","position":12,"is_corresponding":false},{"id":328731,"name":"Andrea Antonini","orcid":"0000-0002-2013-1521","position":0,"is_corresponding":true}],"reference_count":85,"raw_metadata":null,"created_at":"2026-07-18T21:10:11.612909Z","pmid":"33048047","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}