{"doi":"10.7554/elife.58668","title":"Alternative splicing at neuroligin site A regulates glycan interaction and synaptogenic activity","abstract":"Post-transcriptional mechanisms regulating cell surface synaptic organizing complexes that control the properties of connections in brain circuits are poorly understood. Alternative splicing regulates the prototypical synaptic organizing complex, neuroligin-neurexin. In contrast to the well-studied neuroligin splice site B, little is known about splice site A. We discovered that inclusion of the positively charged A1 insert in mouse neuroligin-1 increases its binding to heparan sulphate, a modification on neurexin. The A1 insert increases neurexin recruitment, presynaptic differentiation, and synaptic transmission mediated by neuroligin-1. We propose that the A1 insert could be a target for alleviating the consequences of deleterious NLGN1/3 mutations, supported by assays with the autism-linked neuroligin-1-P89L mutant. An enrichment of neuroligin-1 A1 in GABAergic neuron types suggests a role in synchrony of cortical circuits. Altogether, these data reveal an unusual mode by which neuroligin splicing controls synapse development through protein-glycan interaction and identify it as a potential therapeutic target.","journal":"eLife","year":2020,"id":78193,"datarank":0.6863474894932642,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"self_citation_contribution":0.44166584687496613,"citation_network_contribution":0.24468164261829803,"self_endowment_contribution":0.44166584687496613,"citer_contribution":0.24468164261829803,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":17,"citers_with_citation_signal":15,"citers_with_endowment":15,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.955,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE115746","GSE133291","GSE38497","GSE47966","51733"],"SRA":["SRP055008","SRP142522","SRP128054"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":406988,"name":"Huijuan Feng","orcid":"0000-0002-4005-560X","position":1,"is_corresponding":false},{"id":406989,"name":"Steven A. Connor","orcid":"0000-0002-0198-5134","position":2,"is_corresponding":false},{"id":406990,"name":"Andrea Toledo","orcid":"0000-0002-8310-8636","position":3,"is_corresponding":false},{"id":406991,"name":"Peng Zhang","orcid":"0000-0001-8071-3729","position":4,"is_corresponding":false},{"id":406992,"name":"Yue Zhang","orcid":"0000-0003-2128-8472","position":5,"is_corresponding":false},{"id":406993,"name":"Olivier Thoumine","orcid":"0000-0002-8041-1349","position":6,"is_corresponding":false},{"id":406994,"name":"Chaolin Zhang","orcid":"0000-0002-8310-7537","position":7,"is_corresponding":false},{"id":247161,"name":"Ann Marie Craig","orcid":"0000-0002-8651-8200","position":8,"is_corresponding":false},{"id":406987,"name":"Shinichiro Oku","orcid":"0000-0002-1916-1870","position":0,"is_corresponding":true}],"reference_count":77,"raw_metadata":null,"created_at":"2026-07-18T21:49:40.769098Z","pmid":"32915137","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}