{"doi":"10.7554/elife.58305","title":"Damage-responsive, maturity-silenced enhancers regulate multiple genes that direct regeneration in Drosophila","abstract":"Like tissues of many organisms, Drosophila imaginal discs lose the ability to regenerate as they mature. This loss of regenerative capacity coincides with reduced damage-responsive expression of multiple genes needed for regeneration. We previously showed that two such genes, wg and Wnt6, are regulated by a single damage-responsive enhancer that becomes progressively inactivated via Polycomb-mediated silencing as discs mature (Harris et al., 2016). Here we explore the generality of this mechanism and identify additional damage-responsive, maturity-silenced (DRMS) enhancers, some near genes known to be required for regeneration such as Mmp1, and others near genes that we now show function in regeneration. Using a novel GAL4-independent ablation system we characterize two DRMS-associated genes, apontic (apt), which curtails regeneration and CG9752/asperous (aspr), which promotes it. This mechanism of suppressing regeneration by silencing damage-responsive enhancers at multiple loci can be partially overcome by reducing activity of the chromatin regulator extra sex combs (esc).","journal":"eLife","year":2020,"id":55430,"datarank":1.8530615834775097,"base_score":4.543294782270004,"endowment":4.543294782270004,"self_citation_contribution":0.6814942173405006,"citation_network_contribution":1.171567366137009,"self_endowment_contribution":0.6814942173405006,"citer_contribution":1.171567366137009,"corpus_percentile":null,"corpus_rank":null,"citation_count":93,"citer_count":70,"citers_with_citation_signal":47,"citers_with_endowment":47,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.948,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE140755"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":286472,"name":"Michael J Stinchfield","orcid":"0000-0002-6307-7270","position":1,"is_corresponding":false},{"id":286473,"name":"Spencer L. Nystrom","orcid":"0000-0003-1000-1579","position":2,"is_corresponding":false},{"id":286474,"name":"Daniel J. McKay","orcid":"0000-0001-8226-0604","position":3,"is_corresponding":false},{"id":286475,"name":"Iswar K. Hariharan","orcid":"0000-0001-6505-0744","position":4,"is_corresponding":false},{"id":286471,"name":"Robin E. Harris","orcid":"0000-0001-6945-6741","position":0,"is_corresponding":true}],"reference_count":91,"raw_metadata":null,"created_at":"2026-07-18T21:04:59.506324Z","pmid":"32490812","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}