{"doi":"10.7554/elife.58157","title":"Cryo-EM structure of VASH1-SVBP bound to microtubules","abstract":"The dynamic tyrosination-detyrosination cycle of α-tubulin regulates microtubule functions. Perturbation of this cycle impairs mitosis, neural physiology, and cardiomyocyte contraction. The carboxypeptidases vasohibins 1 and 2 (VASH1 and VASH2), in complex with the small vasohibin-binding protein (SVBP), mediate α-tubulin detyrosination. These enzymes detyrosinate microtubules more efficiently than soluble αβ-tubulin heterodimers. The structural basis for this substrate preference is not understood. Using cryo-electron microscopy (cryo-EM), we have determined the structure of human VASH1-SVBP bound to microtubules. The acidic C-terminal tail of α-tubulin binds to a positively charged groove near the active site of VASH1. VASH1 forms multiple additional contacts with the globular domain of α-tubulin, including contacts with a second α-tubulin in an adjacent protofilament. Simultaneous engagement of two protofilaments by VASH1 can only occur within the microtubule lattice, but not with free αβ heterodimers. These lattice-specific interactions enable preferential detyrosination of microtubules by VASH1.","journal":"eLife","year":2020,"id":92177,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":43,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.954,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":461259,"name":"Yang Li","orcid":"0000-0002-6942-507X","position":1,"is_corresponding":false},{"id":386468,"name":"Xuecheng Ye","orcid":null,"position":2,"is_corresponding":false},{"id":461260,"name":"Haishan Gao","orcid":"0000-0002-4954-8793","position":3,"is_corresponding":false},{"id":461261,"name":"Zhubing Shi","orcid":"0000-0002-9624-4960","position":4,"is_corresponding":false},{"id":246167,"name":"Xuelian Luo","orcid":"0000-0002-5058-4695","position":5,"is_corresponding":false},{"id":57571,"name":"Luke M. Rice","orcid":"0000-0001-6551-3307","position":6,"is_corresponding":false},{"id":461262,"name":"Hongtao Yu","orcid":"0000-0002-8861-049X","position":7,"is_corresponding":false},{"id":461258,"name":"Faxiang Li","orcid":"0000-0002-6442-9063","position":0,"is_corresponding":true}],"reference_count":48,"raw_metadata":null,"created_at":"2026-07-18T22:29:54.256205Z","pmid":"32773040","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}