{"doi":"10.7554/elife.58039","title":"G protein-regulated endocytic trafficking of adenylyl cyclase type 9","abstract":"GPCRs are increasingly recognized to initiate signaling via heterotrimeric G proteins as they move through the endocytic network, but little is known about how relevant G protein effectors are localized. Here we report selective trafficking of adenylyl cyclase type 9 (AC9) from the plasma membrane to endosomes while adenylyl cyclase type 1 (AC1) remains in the plasma membrane, and stimulation of AC9 trafficking by ligand-induced activation of Gs-coupled GPCRs. AC9 transits a similar, dynamin-dependent early endocytic pathway as ligand-activated GPCRs. However, unlike GPCR traffic control which requires β-arrestin but not Gs, AC9 traffic control requires Gs but not β-arrestin. We also show that AC9, but not AC1, mediates cAMP production stimulated by endogenous receptor activation in endosomes. These results reveal dynamic and isoform-specific trafficking of adenylyl cyclase in the endocytic network, and a discrete role of a heterotrimeric G protein in regulating the subcellular distribution of a relevant effector.","journal":"eLife","year":2020,"id":60572,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":61,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9484,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":320023,"name":"Roshanak Irannejad","orcid":"0000-0001-8702-2285","position":1,"is_corresponding":false},{"id":320024,"name":"Tanya A. Baldwin","orcid":"0000-0001-7142-639X","position":2,"is_corresponding":false},{"id":320025,"name":"Aparna Sundaram","orcid":"0000-0002-4076-4756","position":3,"is_corresponding":false},{"id":273870,"name":"J. Silvio Gutkind","orcid":"0000-0002-5150-4482","position":4,"is_corresponding":false},{"id":255551,"name":"Asuka Inoue","orcid":"0000-0003-0805-4049","position":5,"is_corresponding":false},{"id":320026,"name":"Carmen Dessauer","orcid":"0000-0003-1210-4280","position":6,"is_corresponding":false},{"id":320027,"name":"Mark von Zastrow","orcid":"0000-0003-1375-6926","position":7,"is_corresponding":false},{"id":320022,"name":"André M Lazar","orcid":"0000-0003-3372-3742","position":0,"is_corresponding":true}],"reference_count":88,"raw_metadata":null,"created_at":"2026-07-18T21:09:05.493770Z","pmid":"32515353","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}