{"doi":"10.7554/elife.56935","title":"Specific labeling of synaptic schwann cells reveals unique cellular and molecular features","abstract":"Perisynaptic Schwann cells (PSCs) are specialized, non-myelinating, synaptic glia of the neuromuscular junction (NMJ), that participate in synapse development, function, maintenance, and repair. The study of PSCs has relied on an anatomy-based approach, as the identities of cell-specific PSC molecular markers have remained elusive. This limited approach has precluded our ability to isolate and genetically manipulate PSCs in a cell specific manner. We have identified neuron-glia antigen 2 (NG2) as a unique molecular marker of S100β+ PSCs in skeletal muscle. NG2 is expressed in Schwann cells already associated with the NMJ, indicating that it is a marker of differentiated PSCs. Using a newly generated transgenic mouse in which PSCs are specifically labeled, we show that PSCs have a unique molecular signature that includes genes known to play critical roles in PSCs and synapses. These findings will serve as a springboard for revealing drivers of PSC differentiation and function.","journal":"eLife","year":2020,"id":57948,"datarank":2.353164839151827,"base_score":4.330733340286331,"endowment":4.330733340286331,"self_citation_contribution":0.6496100010429497,"citation_network_contribution":1.7035548381088774,"self_endowment_contribution":0.6496100010429497,"citer_contribution":1.7035548381088774,"corpus_percentile":null,"corpus_rank":null,"citation_count":75,"citer_count":65,"citers_with_citation_signal":58,"citers_with_endowment":58,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9573,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE152774"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":302890,"name":"Thomas Taetzsch","orcid":"0000-0003-3257-1142","position":1,"is_corresponding":false},{"id":302891,"name":"Sydney V. Doss","orcid":"0000-0002-3427-4654","position":2,"is_corresponding":false},{"id":302892,"name":"Kerilyn Godbe","orcid":"0000-0002-3846-7533","position":3,"is_corresponding":false},{"id":302893,"name":"John C. Chappell","orcid":"0000-0002-0427-5170","position":4,"is_corresponding":false},{"id":302894,"name":"Robert E. Settlage","orcid":"0000-0002-1354-7609","position":5,"is_corresponding":false},{"id":302895,"name":"Gregorio Valdez","orcid":"0000-0002-0375-4532","position":6,"is_corresponding":false},{"id":302889,"name":"Ryan Castro","orcid":"0000-0002-2316-8039","position":0,"is_corresponding":true}],"reference_count":103,"raw_metadata":null,"created_at":"2026-07-18T21:06:48.611399Z","pmid":"32584256","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}