{"doi":"10.7554/elife.54031","title":"Structure of the AAA protein Msp1 reveals mechanism of mislocalized membrane protein extraction","abstract":"The AAA protein Msp1 extracts mislocalized tail-anchored membrane proteins and targets them for degradation, thus maintaining proper cell organization. How Msp1 selects its substrates and firmly engages them during the energetically unfavorable extraction process remains a mystery. To address this question, we solved cryo-EM structures of Msp1-substrate complexes at near-atomic resolution. Akin to other AAA proteins, Msp1 forms hexameric spirals that translocate substrates through a central pore. A singular hydrophobic substrate recruitment site is exposed at the spiral's seam, which we propose positions the substrate for entry into the pore. There, a tight web of aromatic amino acids grips the substrate in a sequence-promiscuous, hydrophobic milieu. Elements at the intersubunit interfaces coordinate ATP hydrolysis with the subunits' positions in the spiral. We present a comprehensive model of Msp1's mechanism, which follows general architectural principles established for other AAA proteins yet specializes Msp1 for its unique role in membrane protein extraction.","journal":"eLife","year":2020,"id":58966,"datarank":2.0571791961898778,"base_score":4.248495242049359,"endowment":4.248495242049359,"self_citation_contribution":0.637274286307404,"citation_network_contribution":1.4199049098824739,"self_endowment_contribution":0.637274286307404,"citer_contribution":1.4199049098824739,"corpus_percentile":null,"corpus_rank":null,"citation_count":69,"citer_count":66,"citers_with_citation_signal":55,"citers_with_endowment":55,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9536,"is_data_producer":true,"deposit_databanks":{"PDB":["6PE0","6PDW","6PDY"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":138179,"name":"Alexander G. Myasnikov","orcid":null,"position":1,"is_corresponding":false},{"id":297511,"name":"Xingjie Pan","orcid":"0000-0002-0060-4352","position":2,"is_corresponding":false},{"id":105723,"name":"Peter Walter","orcid":"0000-0002-6849-708X","position":3,"is_corresponding":false},{"id":309379,"name":"Lan Wang","orcid":"0000-0002-8931-7201","position":0,"is_corresponding":true}],"reference_count":60,"raw_metadata":null,"created_at":"2026-07-18T21:07:33.416320Z","pmid":"31999255","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}