{"doi":"10.7554/elife.53558","title":"Chromosome organization by one-sided and two-sided loop extrusion","abstract":"SMC complexes, such as condensin or cohesin, organize chromatin throughout the cell cycle by a process known as loop extrusion. SMC complexes reel in DNA, extruding and progressively growing DNA loops. Modeling assuming two-sided loop extrusion reproduces key features of chromatin organization across different organisms. In vitro single-molecule experiments confirmed that yeast condensins extrude loops, however, they remain anchored to their loading sites and extrude loops in a 'one-sided' manner. We therefore simulate one-sided loop extrusion to investigate whether 'one-sided' complexes can compact mitotic chromosomes, organize interphase domains, and juxtapose bacterial chromosomal arms, as can be done by 'two-sided' loop extruders. While one-sided loop extrusion cannot reproduce these phenomena, variants can recapitulate in vivo observations. We predict that SMC complexes in vivo constitute effectively two-sided motors or exhibit biased loading and propose relevant experiments. Our work suggests that loop extrusion is a viable general mechanism of chromatin organization.","journal":"eLife","year":2020,"id":51200,"datarank":3.4174715003938534,"base_score":5.10594547390058,"endowment":5.10594547390058,"self_citation_contribution":0.7658918210850871,"citation_network_contribution":2.6515796793087665,"self_endowment_contribution":0.7658918210850871,"citer_contribution":2.6515796793087665,"corpus_percentile":null,"corpus_rank":null,"citation_count":164,"citer_count":100,"citers_with_citation_signal":100,"citers_with_endowment":100,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9397,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE96107","GSE68418"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":254910,"name":"Aafke A. van den Berg","orcid":"0000-0003-2991-9478","position":1,"is_corresponding":false},{"id":135507,"name":"Hugo B. Brandão","orcid":"0000-0001-5496-0638","position":2,"is_corresponding":false},{"id":254911,"name":"John F. Marko","orcid":"0000-0003-4151-9530","position":3,"is_corresponding":false},{"id":109520,"name":"Leonid A. Mirny","orcid":"0000-0002-0785-5410","position":4,"is_corresponding":false},{"id":250608,"name":"Edward J. Banigan","orcid":"0000-0001-5478-7425","position":0,"is_corresponding":true}],"reference_count":174,"raw_metadata":null,"created_at":"2026-07-18T20:40:28.278215Z","pmid":"32250245","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}