{"doi":"10.7554/elife.52786","title":"Building customizable auto-luminescent luciferase-based reporters in plants","abstract":"Bioluminescence is a powerful biological signal that scientists have repurposed as a reporter for gene expression in plants and animals. However, there are downsides associated with the need to provide a substrate to these reporters, including its high cost and non-uniform tissue penetration. In this work we reconstitute a fungal bioluminescence pathway (FBP) in planta using a composable toolbox of parts. We demonstrate that the FBP can create luminescence across various tissues in a broad range of plants without external substrate addition. We also show how our toolbox can be used to deploy the FBP in planta to build auto-luminescent reporters for the study of gene-expression and hormone fluxes. A low-cost imaging platform for gene expression profiling is also described. These experiments lay the groundwork for future construction of programmable auto-luminescent plant traits, such as light driven plant-pollinator interactions or light emitting plant-based sensors.","journal":"eLife","year":2020,"id":56302,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":86,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.956,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":292373,"name":"Colby G. Starker","orcid":"0000-0002-6774-7227","position":1,"is_corresponding":false},{"id":292374,"name":"James C. Chamness","orcid":"0000-0001-8862-0236","position":2,"is_corresponding":false},{"id":292375,"name":"Nayoung Lee","orcid":"0000-0002-0353-6455","position":3,"is_corresponding":false},{"id":293719,"name":"Sydney Stokke","orcid":null,"position":4,"is_corresponding":false},{"id":293720,"name":"Cecily Wang","orcid":null,"position":5,"is_corresponding":false},{"id":292376,"name":"Ryan Swanson","orcid":"0009-0005-6389-2215","position":6,"is_corresponding":false},{"id":293721,"name":"Furva Rizvi","orcid":null,"position":7,"is_corresponding":false},{"id":292377,"name":"Takato Imaizumi","orcid":"0000-0001-9396-4412","position":8,"is_corresponding":false},{"id":292378,"name":"Daniel F. Voytas","orcid":"0000-0002-4944-1224","position":9,"is_corresponding":false},{"id":292372,"name":"Arjun Khakhar","orcid":"0000-0002-4676-6533","position":0,"is_corresponding":true}],"reference_count":31,"raw_metadata":null,"created_at":"2026-07-18T21:05:49.581291Z","pmid":"32209230","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}