{"doi":"10.7554/elife.51898","title":"Structural insights into mRNA reading frame regulation by tRNA modification and slippery codon–anticodon pairing","abstract":"Modifications in the tRNA anticodon loop, adjacent to the three-nucleotide anticodon, influence translation fidelity by stabilizing the tRNA to allow for accurate reading of the mRNA genetic code. One example is the N1-methylguanosine modification at guanine nucleotide 37 (m 1 G37) located in the anticodon loop andimmediately adjacent to the anticodon nucleotides 34, 35, 36. The absence of m 1 G37 in tRNA Pro causes +1 frameshifting on polynucleotide, slippery codons. Here, we report structures of the bacterial ribosome containing tRNA Pro bound to either cognate or slippery codons to determine how the m 1 G37 modification prevents mRNA frameshifting. The structures reveal that certain codon–anticodon contexts and the lack of m 1 G37 destabilize interactions of tRNA Pro with the P site of the ribosome, causing large conformational changes typically only seen during EF-G-mediated translocation of the mRNA-tRNA pairs. These studies provide molecular insights into how m 1 G37 stabilizes the interactions of tRNA Pro with the ribosome in the context of a slippery mRNA codon.","journal":"eLife","year":2020,"id":91882,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":44,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9612,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":459763,"name":"Samuel Hong","orcid":"0000-0002-6870-6857","position":1,"is_corresponding":false},{"id":460471,"name":"S. Sunita","orcid":null,"position":2,"is_corresponding":false},{"id":459764,"name":"Tatsuya Maehigashi","orcid":"0000-0002-9639-2250","position":3,"is_corresponding":false},{"id":351136,"name":"Ruben L. Gonzalez","orcid":"0000-0002-1344-5581","position":4,"is_corresponding":false},{"id":459765,"name":"Paul C. Whitford","orcid":"0000-0001-7104-2265","position":5,"is_corresponding":false},{"id":17817,"name":"Christine M. Dunham","orcid":"0000-0002-8821-688X","position":6,"is_corresponding":false},{"id":459762,"name":"Eric D. Hoffer","orcid":"0000-0002-2273-6743","position":0,"is_corresponding":true}],"reference_count":64,"raw_metadata":null,"created_at":"2026-07-18T22:29:21.932251Z","pmid":"33016876","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}