{"doi":"10.7554/elife.51796","title":"A MAC2-positive progenitor-like microglial population is resistant to CSF1R inhibition in adult mouse brain","abstract":"Microglia are the resident myeloid cells in the central nervous system (CNS). The majority of microglia rely on CSF1R signaling for survival. However, a small subset of microglia in mouse brains can survive without CSF1R signaling and reestablish the microglial homeostatic population after CSF1R signaling returns. Using single-cell transcriptomic analysis, we characterized the heterogeneous microglial populations under CSF1R inhibition, including microglia with reduced homeostatic markers and elevated markers of inflammatory chemokines and proliferation. Importantly, MAC2/ Lgals3 was upregulated under CSF1R inhibition, and shared striking similarities with microglial progenitors in the yolk sac and immature microglia in early embryos. Lineage-tracing studies revealed that these MAC2+ cells were of microglial origin. MAC2+ microglia were also present in non-treated adult mouse brains and exhibited immature transcriptomic signatures indistinguishable from those that survived CSF1R inhibition, supporting the notion that MAC2+ progenitor-like cells are present among adult microglia.","journal":"eLife","year":2020,"id":56891,"datarank":2.5164730493874266,"base_score":4.418840607796598,"endowment":4.418840607796598,"self_citation_contribution":0.6628260911694899,"citation_network_contribution":1.8536469582179367,"self_endowment_contribution":0.6628260911694899,"citer_contribution":1.8536469582179367,"corpus_percentile":null,"corpus_rank":null,"citation_count":82,"citer_count":78,"citers_with_citation_signal":72,"citers_with_endowment":72,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9416,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE150169"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":295940,"name":"Li Fan","orcid":"0000-0003-1780-6919","position":1,"is_corresponding":false},{"id":295941,"name":"Lay Kodama","orcid":"0000-0001-8419-8445","position":2,"is_corresponding":false},{"id":112571,"name":"Peter Sohn","orcid":null,"position":3,"is_corresponding":false},{"id":270692,"name":"Man Ying Wong","orcid":"0000-0002-2165-9328","position":4,"is_corresponding":false},{"id":295942,"name":"Gergey Alzaem Mousa","orcid":"0000-0002-7208-5803","position":5,"is_corresponding":false},{"id":297016,"name":"Yungui Zhou","orcid":null,"position":6,"is_corresponding":false},{"id":275681,"name":"Yaqiao Li","orcid":"0000-0002-9043-0375","position":7,"is_corresponding":false},{"id":250399,"name":"Li Gan","orcid":"0000-0003-4600-275X","position":8,"is_corresponding":false},{"id":295939,"name":"Lihong Zhan","orcid":"0000-0001-7772-9340","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":null,"created_at":"2026-07-18T21:06:09.605575Z","pmid":"33054973","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}