{"doi":"10.7554/elife.51576","title":"Functionally heterogeneous human satellite cells identified by single cell RNA sequencing","abstract":"Although heterogeneity is recognized within the murine satellite cell pool, a comprehensive understanding of distinct subpopulations and their functional relevance in human satellite cells is lacking. We used a combination of single cell RNA sequencing and flow cytometry to identify, distinguish, and physically separate novel subpopulations of human PAX7+ satellite cells (Hu-MuSCs) from normal muscles. We found that, although relatively homogeneous compared to activated satellite cells and committed progenitors, the Hu-MuSC pool contains clusters of transcriptionally distinct cells with consistency across human individuals. New surface marker combinations were enriched in transcriptional subclusters, including a subpopulation of Hu-MuSCs marked by CXCR4/CD29/CD56/CAV1 (CAV1+). In vitro, CAV1+ Hu-MuSCs are morphologically distinct, and characterized by resistance to activation compared to CAV1- Hu-MuSCs. In vivo, CAV1+ Hu-MuSCs demonstrated increased engraftment after transplantation. Our findings provide a comprehensive transcriptional view of normal Hu-MuSCs and describe new heterogeneity, enabling separation of functionally distinct human satellite cell subpopulations.","journal":"eLife","year":2020,"id":51594,"datarank":0.7545656882088654,"base_score":5.030437921392435,"endowment":5.030437921392435,"self_citation_contribution":0.7545656882088654,"citation_network_contribution":0.0,"self_endowment_contribution":0.7545656882088654,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":152,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9354,"is_data_producer":true,"deposit_databanks":{"Dryad":["10.7272/Q65X273X"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":258285,"name":"Steven Garcia","orcid":"0000-0002-7833-6677","position":1,"is_corresponding":false},{"id":258286,"name":"Katharine Striedinger","orcid":"0000-0002-9071-4810","position":2,"is_corresponding":false},{"id":261005,"name":"Jake Wu","orcid":null,"position":3,"is_corresponding":false},{"id":258287,"name":"Solomon Lee","orcid":"0000-0002-1230-3741","position":4,"is_corresponding":false},{"id":86162,"name":"Lauren E. Byrnes","orcid":"0000-0001-5991-8201","position":5,"is_corresponding":false},{"id":258288,"name":"Alvin Wong","orcid":"0000-0001-8775-1779","position":6,"is_corresponding":false},{"id":261006,"name":"Xuefeng Sun","orcid":null,"position":7,"is_corresponding":false},{"id":261007,"name":"Stanley Tamaki","orcid":null,"position":8,"is_corresponding":false},{"id":258289,"name":"Andrew S. Brack","orcid":"0000-0002-8798-7084","position":9,"is_corresponding":false},{"id":258290,"name":"Jason H. Pomerantz","orcid":"0000-0002-5107-1883","position":10,"is_corresponding":false},{"id":258284,"name":"Emilie Barruet","orcid":"0000-0002-4593-024X","position":0,"is_corresponding":true}],"reference_count":109,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T20:41:30.009880Z","pmid":"32234209","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}