{"doi":"10.7554/elife.107732.2","title":"A three-dimensional immunofluorescence atlas of the brain of the hackled-orb weaver spider, Uloborus diversus","abstract":"Spider orb-web building is a captivating, rare example of animal construction, whose neural underpinnings remain undiscovered. We created a three-dimensional atlas for the hackled orb-weaver, Uloborus diversus, based on immunostaining for the presynaptic component, synapsin, in whole-mounted spider synganglia. Whereas spider neuroanatomy has thus far been most comprehensively studied in cursorial species, this optically-sectioned atlas contributes a continuous, finely resolved model of the central nervous system of an orb-web building spider. Aligned to this volume, we examined the expression patterns of neuronal populations representing many of the classical neurotransmitters and neuromodulators (GABA, Acetylcholine, Serotonin, Octopamine/Tyramine), as well as a subset of neuropeptides (AllatostatinA, Crustacean Cardioactive Peptide (CCAP), FMRFamide, Proctolin) – detailing immunoreactivity in an unbiased fashion throughout the synganglion to reveal co-expression in known structures (such as the arcuate body), as well as novel neuropils not readily apparent in prior spider research, including the tonsillar neuropil as well as a potential protocerebral bridge. These structures provide targets for future functional studies, and taken together could represent a spider equivalent of the central complex, contributing to behaviors such as web-building.","journal":"eLife","year":2025,"id":586567,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":0.0,"corpus_rank":10062,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":true,"is_dataset_confidence":0.775,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":673664,"name":"Abel Corver","orcid":"0000-0003-1431-9832","position":1,"is_corresponding":false},{"id":673666,"name":"Andrew Gordus","orcid":"0000-0002-5550-0286","position":2,"is_corresponding":false},{"id":347402,"name":"Gregory Artiushin","orcid":"0000-0003-1615-3012","position":0,"is_corresponding":true}],"reference_count":80,"raw_metadata":null,"created_at":"2026-07-19T02:59:32.191237Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}