{"doi":"10.7554/elife.106910.2.sa4","title":"Author response: Altair-LSFM: A High-Resolution, Easy-to-Build Light-Sheet Microscope for Sub-Cellular Imaging","abstract":"Although several open-source, easy-to-assemble light-sheet microscope platforms already exist—such as mesoSPIM, OpenSPIM, and OpenSpin—they are optimized for imaging large specimens and lack the resolution required to visualize sub-cellular features, such as organelles or cytoskeletal architectures. In contrast, Latice Light-Sheet Microscopy (LLSM) achieves the resolution necessary to resolve such fine structures but, in its open-source implementation, can be alignment- and maintenance-intensive, often requiring specialist expertise. To address this gap, we developed Altair-LSFM, a high-resolution, open-source, sample-scanning light-sheet microscope specifically designed for sub-cellular imaging. By optimizing the optical pathway in silico, we created a custom baseplate that greatly simplifies alignment and assembly. The system integrates streamlined optoelectronics and optomechanics with seamless operation through our open-source software, navigate. Altair-LSFM achieves lateral and axial resolutions of approximately 235 nm and 350 nm, respectively, across a 266-micron field of view after deconvolution. We validate the system’s capabilities by imaging sub-diffraction fluorescent nanospheres and visualizing fine structural details in mammalian cells, including microtubules, actin filaments, nuclei, and Golgi apparatus. We further demonstrate its live-cell imaging capabilities by visualizing microtubules and vimentin intermediate filaments in actively migrating cells.","journal":null,"year":2025,"id":587039,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9524,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1213274,"name":"Seweryn Gałecki","orcid":"0000-0002-7020-0804","position":1,"is_corresponding":false},{"id":1358832,"name":"Hsin-Yu Lin","orcid":null,"position":2,"is_corresponding":false},{"id":282884,"name":"Xiaoding Wang","orcid":"0000-0003-2333-2270","position":3,"is_corresponding":false},{"id":1502581,"name":"Kevin M Dean","orcid":null,"position":4,"is_corresponding":false},{"id":1268360,"name":"John Haug","orcid":"0000-0001-8247-3271","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:59:36.020030Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}