{"doi":"10.7554/elife.105062.1","title":"Single-molecule imaging reveals the role of membrane-binding motif and C-terminal domain of RNase E in its localization and diffusion in Escherichia coli","abstract":"Abstract In Escherichia coli, RNase E is the key enzyme for RNA processing and mRNA degradation. Despite the conserved function across bacteria, the domain composition of RNase E varies significantly among species, possibly affecting the enzyme’s subcellular localization, mobility, and function. In this work, we used super-resolution microscopy to find that 93% of RNase E is localized to the membrane in E. coli and exhibits slow diffusion comparable to polysomes diffusing in the cytoplasm. By replacing the native amphipathic membrane targeting sequence (MTS) with a transmembrane motif, we discovered that the MTS results in slower diffusion and stronger membrane binding than a transmembrane motif. Additionally, the evolutionarily divergent C-terminal domain (CTD) was shown to grant slow diffusion of RNase E but to weaken its membrane binding. By analyzing how membrane localization and diffusion of RNase E affect mRNA degradation rates in vivo, we provide new insights into RNase E’s role in the spatiotemporal organization of RNA processes in bacterial cells.","journal":"eLife","year":2025,"id":557205,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9543,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1328822,"name":"Yu-Huan Wang","orcid":"0009-0000-3086-1336","position":1,"is_corresponding":false},{"id":1329299,"name":"Shobhna Shobhna","orcid":null,"position":2,"is_corresponding":false},{"id":1328823,"name":"Seunghyeon Kim","orcid":"0009-0000-5450-8860","position":3,"is_corresponding":false},{"id":1329301,"name":"Jeechul Woo","orcid":null,"position":4,"is_corresponding":false},{"id":98027,"name":"Emad Tajkhorshid","orcid":"0000-0001-8434-1010","position":5,"is_corresponding":false},{"id":717705,"name":"Sang‐Jin Kim","orcid":"0000-0002-8444-7891","position":6,"is_corresponding":false},{"id":1328821,"name":"Laura Troyer","orcid":"0000-0002-7208-6654","position":0,"is_corresponding":true}],"reference_count":69,"raw_metadata":null,"created_at":"2026-07-19T02:55:17.435961Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}