{"doi":"10.7554/elife.101894","title":"Calcium transfer from the ER to other organelles for optimal signaling in Toxoplasma gondii","abstract":"Ca 2+ signaling in cells begins with the opening of Ca 2+ channels in either the plasma membrane (PM) or endoplasmic reticulum (ER), leading to a sharp increase in the physiologically low (<100 nM) cytosolic Ca 2+ level. The temporal and spatial regulation of Ca² + is crucial for the precise activation of key biological processes. In the apicomplexan parasite Toxoplasma gondii , which infects approximately one-third of the global population, Ca² + signaling governs essential aspects of the parasite’s infection cycle. T. gondii relies on Ca² + signals to regulate pathogenic traits, with several Ca² + -signaling components playing critical roles. Ca 2+ entry from the extracellular environment has been demonstrated in T. gondii for both, extracellular parasites, exposed to high Ca 2+ , and intracellular parasites, which acquire Ca² + from host cells during host Ca² + signaling events. Active egress, an essential step of the parasite’s infection cycle, is preceded by a large increase in cytosolic Ca 2+ , most likely initiated by release from intracellular stores. However, extracellular Ca 2+ is also necessary to reach a cytosolic Ca 2+ threshold required for timely egress. In this study, we investigated the mechanism of intracellular Ca² + store replenishment and identified a central role for the SERCA-Ca 2+ -ATPase in maintaining Ca² + homeostasis within the ER and in other organelles. We demonstrate mitochondrial Ca 2+ uptake, which occurs by transfer of Ca 2+ from the ER, likely through membrane contact sites. Our findings suggest that the T. gondii ER plays a key role in sequestering and redistributing Ca² + to intracellular organelles following Ca² + influx at the PM.","journal":"eLife","year":2024,"id":487556,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9491,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":379320,"name":"Beejan Asady","orcid":"0000-0002-7503-418X","position":1,"is_corresponding":false},{"id":1331887,"name":"Le Chang","orcid":"0000-0002-6154-7304","position":2,"is_corresponding":false},{"id":573821,"name":"Miryam Andrea Hortua Triana","orcid":"0000-0001-5964-8512","position":3,"is_corresponding":false},{"id":1331888,"name":"Catherine Li","orcid":"0009-0009-5954-9288","position":4,"is_corresponding":false},{"id":353145,"name":"Isabelle Coppens","orcid":"0000-0001-5549-2362","position":5,"is_corresponding":false},{"id":480891,"name":"Silvia N.J. Moreno","orcid":"0000-0002-2041-6295","position":6,"is_corresponding":false},{"id":480885,"name":"Zhu‐Hong Li","orcid":"0000-0003-4940-0729","position":0,"is_corresponding":true}],"reference_count":78,"raw_metadata":null,"created_at":"2026-07-19T02:08:10.215754Z","pmid":"41222462","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}