{"doi":"10.7287/peerj.preprints.904v2","title":"Perilipin-related protein regulates lipid metabolism in\n                  <i>C. elegans</i>","abstract":"<jats:p>\n                  The perilipins are lipid droplet surface proteins that contribute to fat metabolism by controlling the access of lipids to lipolytic enzymes. Perilipins have been identified in organisms as diverse as metazoa, fungi, and amoebas but strikingly not in nematodes. Here we identify the protein encoded by the\n                  <jats:italic>W01A8.1</jats:italic>\n                  gene in\n                  <jats:italic>Caenorhabditis elegans</jats:italic>\n                  as the closest homologue of metazoan perilipin. We demonstrate that nematode W01A8.1 is a cytoplasmic protein residing on lipid droplets. Human perilipins 1 and 2 localize in transgenic\n                  <jats:italic>C. elegans</jats:italic>\n                  on the same structures as proteins expressed from W01A8.1 gene. Inhibition and elimination of W01A8.1 affects the appearance of lipid droplets especially visible as the formation of large lipid droplets localized around the dividing nucleus during the early zygotic divisions. This phenomenon disappears in later stages of embryogenesis indicating the existence of an additional mechanism of lipid regulation in\n                  <jats:italic>C. elegans</jats:italic>\n                  . Our results demonstrate that perilipin-related regulation of fat metabolism is conserved in nematodes and provide new possibilities for functional studies of lipid metabolism.\n                </jats:p>","journal":null,"year":null,"id":671972,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1750496,"name":"Filip Kaššák","orcid":null,"position":1,"is_corresponding":false},{"id":1750497,"name":"Markéta Kostrouchová","orcid":null,"position":2,"is_corresponding":false},{"id":1043138,"name":"Jan Novotný","orcid":"0000-0001-6021-0577","position":3,"is_corresponding":false},{"id":1750499,"name":"Michael W. Krause","orcid":null,"position":4,"is_corresponding":false},{"id":1750500,"name":"Vladimír Saudek","orcid":null,"position":5,"is_corresponding":false},{"id":1750501,"name":"Zdenek Kostrouch","orcid":null,"position":6,"is_corresponding":false},{"id":1750502,"name":"Marta Kostrouchová","orcid":null,"position":7,"is_corresponding":false},{"id":1750495,"name":"Ahmed A. Chughtai","orcid":"0000-0002-9635-7463","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Perilipin-related protein regulates lipid metabolism in\n                  <i>C. elegans</i>","abstract":"<jats:p>\n                  The perilipins are lipid droplet surface proteins that contribute to fat metabolism by controlling the access of lipids to lipolytic enzymes. Perilipins have been identified in organisms as diverse as metazoa, fungi, and amoebas but strikingly not in nematodes. Here we identify the protein encoded by the\n                  <jats:italic>W01A8.1</jats:italic>\n                  gene in\n                  <jats:italic>Caenorhabditis elegans</jats:italic>\n                  as the closest homologue of metazoan perilipin. We demonstrate that nematode W01A8.1 is a cytoplasmic protein residing on lipid droplets. Human perilipins 1 and 2 localize in transgenic\n                  <jats:italic>C. elegans</jats:italic>\n                  on the same structures as proteins expressed from W01A8.1 gene. Inhibition and elimination of W01A8.1 affects the appearance of lipid droplets especially visible as the formation of large lipid droplets localized around the dividing nucleus during the early zygotic divisions. This phenomenon disappears in later stages of embryogenesis indicating the existence of an additional mechanism of lipid regulation in\n                  <jats:italic>C. elegans</jats:italic>\n                  . Our results demonstrate that perilipin-related regulation of fat metabolism is conserved in nematodes and provide new possibilities for functional studies of lipid metabolism.\n                </jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19162232","pmcid":null,"openalex_id":"https://openalex.org/W4231509996","authors":[],"funders":[{"funder_name":"Wellcome Trust","grant_id":"unidentified","title":"unidentified"}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.7287/peerj.preprints.904v2","host_type":""},{"url":"https://doi.org/10.7287/peerj.preprints.904v2","host_type":""},{"url":"https://peerj.com/preprints/904v2.pdf","host_type":"publisher"},{"url":"https://peerj.com/preprints/904v2.xml","host_type":"publisher"},{"url":"https://peerj.com/preprints/904v2.html","host_type":"publisher"},{"url":"https://peerj.com/preprints/904.pdf","host_type":"Unpaywall"},{"url":"https://doi.org/10.7287/peerj.preprints.904v1","host_type":""},{"url":"https://doi.org/10.7717/peerj.1213","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/26357594","host_type":""},{"url":"http://dx.doi.org/10.7717/peerj.1213","host_type":""},{"url":"https://doaj.org/article/e4b114b8d6104de2b47a9822007d6588","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/26357594/","host_type":""},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC4562238/","host_type":""},{"url":"https://dx.doi.org/10.7717/peerj.1213","host_type":""},{"url":"https://archive-ouverte.unige.ch/unige:171921","host_type":""}],"fields_of_study":["Lipid metabolism and biosynthesis","Photosynthetic Processes and Mechanisms","Enzyme Catalysis and Immobilization","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Caenorhabditis elegans","Perilipin","Lipid droplet","Lipid metabolism","Biology","Cell biology","Gene","Cytoplasm","Biochemistry","Lipolysis","QH301-705.5","Bioinformatics","R","Medicine","Perilipin-related protein in C. elegans","Biology (General)","Lipid droplets","Fat metabolism"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T05:39:54.912278Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}